Correlation between photoreceptor injury-regeneration and behavior in a zebrafish model.
Correlation between photoreceptor injury-regeneration and behavior in a zebrafish model.
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斑马鱼模型中光感受器损伤再生与行为之间的相关性。
DOI：
10.4103/1673-5374.206651
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发表时间：
2017-05
影响因子：
6.1
通讯作者：
Li YH
中科院分区：
文献类型：
--
作者：
Wang YJ;Cai SJ;Cui JL;Chen Y;Tang X;Li YH
直接暴露在强烈的可见光下会导致日光性视网膜病变，包括黄斑损伤。症状和体征包括中央暗点、视物变形和视力下降。然而，在细胞水平上研究强光刺激引起的视网膜损伤的研究很少。斑马鱼光感受器的神经网络排列和基因表达模式与在人类中观察到的相似，斑马鱼光感受器损伤可以诱导基于干细胞的细胞再生。因此，斑马鱼视网膜被认为是研究人类光感受器损伤的有用模型。在目前的研究中，斑马鱼的中央视网膜光感受器被选择性地用高强度光刺激来消融。用免疫组织化学和原位杂交法检测损伤后1、3、7d视锥细胞和视杆细胞的视网膜损伤、细胞增殖和再生情况。此外，还使用了明/暗盒子测试来评估斑马鱼的行为。结果表明，光诱导损伤后7d，光感受器开始再生。然而，再生的细胞在病变部位显示出排列紊乱。在损伤-再生过程中，斑马鱼的运动能力降低，趋光性减弱，运动角速度增加。这些行为在许多方面与视网膜损伤和再生的形态变化相匹配。这项研究表明斑马鱼的视网膜具有很强的再生能力。高强度光刺激后的视觉损伤和应激反应似乎有助于行为的改变。
Direct exposure to intensive visible light can lead to solar retinopathy, including macular injury. The signs and symptoms include central scotoma, metamorphopsia, and decreased vision. However, there have been few studies examining retinal injury due to intensive light stimulation at the cellular level. Neural network arrangements and gene expression patterns in zebrafish photoreceptors are similar to those observed in humans, and photoreceptor injury in zebrafish can induce stem cell-based cellular regeneration. Therefore, the zebrafish retina is considered a useful model for studying photoreceptor injury in humans. In the current study, the central retinal photoreceptors of zebrafish were selectively ablated by stimulation with high-intensity light. Retinal injury, cell proliferation and regeneration of cones and rods were assessed at 1, 3 and 7 days post lesion with immunohistochemistry and in situ hybridization. Additionally, a light/dark box test was used to assess zebrafish behavior. The results revealed that photoreceptors were regenerated by 7 days after the light-induced injury. However, the regenerated cells showed a disrupted arrangement at the lesion site. During the injury-regeneration process, the zebrafish exhibited reduced locomotor capacity, weakened phototaxis and increased movement angular velocity. These behaviors matched the morphological changes of retinal injury and regeneration in a number of ways. This study demonstrates that the zebrafish retina has a robust capacity for regeneration. Visual impairment and stress responses following high-intensity light stimulation appear to contribute to the alteration of behaviors.