CELL-SIZE AS A DETERMINANT OF THE CLONE-FORMING ABILITY OF HUMAN KERATINOCYTES
CELL-SIZE AS A DETERMINANT OF THE CLONE-FORMING ABILITY OF HUMAN KERATINOCYTES
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DOI：
10.1073/pnas.82.16.5390
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
GREEN, H
中科院分区：
文献类型：
--
作者：
BARRANDON, Y;GREEN, H
从人表皮分离并随后培养的角质形成细胞如果直径为11 μ m或更小则可形成克隆，但如果直径为12 μ m或更大则不可逆地进一步扩大和终末分化。当11 μ m或更小的初始细胞在培养物中形成快速生长的小克隆时，该克隆的细胞能够发现新的集落，即使它们的直径大至20 μ m。随着克隆体变大和生长变慢，其克隆形成细胞的最大尺寸向表皮减小。直径高达20 μ m的培养细胞在分裂时可产生比其自身小的克隆子代，从而逆转扩大过程。大于20 μ m的细胞不能分裂，因此不能从终末分化中拯救。当角质形成细胞快速繁殖时，它们可逆地将它们能够产生克隆的最大尺寸扩展到通常具有终末分化细胞特征的范围内。这种机制可能使角质形成细胞能够适应增加的增殖速率，以满足其在终末分化期间达到大尺寸的需要。
Keratinocytes isolated from human epidermis and subsequently cultured may form clones if they are 11 .mu.m or less in diameter but are irreversibly committed to further enlargement and terminal differentiation if they are 12 .mu.m or more in diameter. When a founding cell of 11 .mu.m or less forms a small rapidly growing clone in culture, the cells of that clone are able to found new colonies even when their diameter is as great as 20 .mu.m. As the clone becomes larger and grows more slowly, the maximal size of its clonogenic cells is reduced toward that of the epidermis. A cultured cell of up to 20 .mu.m in diameter can, when it divides, give rise to clonogenic progeny smaller than itself, thus reversing the process of enlargement. Cells larger than 20 .mu.m cannot divide and therefore cannot be rescued from terminal differentiation. When keratinocytes multiply rapidly, they extend reversibly the maximal size at which they are capable of generating clones into the range usually chacteristic of terminally differentiating cells. This mechanism may enable the keratinocyte to accommodate an increased rate of multiplication to its need to attain a large size during terminal differentiation.