Near-infrared fluorescence imaging-guided surgery improves recurrence-free survival rate in novel orthotopic animal model of head and neck squamous cell carcinoma
Near-infrared fluorescence imaging-guided surgery improves recurrence-free survival rate in novel orthotopic animal model of head and neck squamous cell carcinoma
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DOI：
10.1002/hed.23980
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发表时间：
2016-04-01
影响因子：
2.9
通讯作者：
Righini, Christian Adrien
中科院分区：
文献类型：
--
作者：
Atallah, Ihab;Milet, Clement;Righini, Christian Adrien
背景资料。需要合适的动物模型来测试头颈部鳞状细胞癌(HNSCC)的新治疗方法，如近红外(NIR)成像引导手术。我们建立了一种最佳的原位HNSCC动物模型(在雌性裸鼠体内)，延长了存活时间。植入后30天，在使用或不使用小型临床级近红外光学成像设备的辅助下，在系统地注射以α(V)β(3)整合素为靶点的基于RGD的荧光探针后，对原位肿瘤进行切除。NIR光学成像引导手术通过检测微小到185 mm的荧光癌残留物，使复发生存率提高了50%；如果完全在无辅助视觉引导下进行切除，这些残留物可能仍然无法识别。近红外光学成像引导手术显示，在我们优化的原位动物模型中，HNSCC肿瘤切除质量得到了改善。(C)2015年威利期刊公司。
Background. Appropriate animal models are required to test novel therapeutics for head and neck squamous cell carcinoma (HNSCC) such as near-infrared (NIR) imaging-guided surgery.Methods. We developed an optimized animal model of orthotopic HNSCC (in female athymic NMRI (Naval Medical Research Institute) nude mice) with a prolonged survival time. Resection of the orthotopic tumors was performed 30 days after implantation with or without the aid of a miniaturized clinical grade NIR optical imaging device, after systemic administration of a fluorescent RGD-based probe that targets alpha(v)beta(3) integrin.Results. NIR optical imaging-guided surgery increased the recurrencefree survival rate by 50% through the detection of fluorescent cancer residues as small as 185 mm; these fragments could remain unidentified if resection was performed exclusively under unaided visual guidance.Conclusion. NIR optical imaging-guided surgery showed an improved HNSCC tumor resection quality in our optimized orthotopic animal model. (C) 2015 Wiley Periodicals, Inc.