Leptospiral immunoglobulin-like proteins elicit protective immunity
Leptospiral immunoglobulin-like proteins elicit protective immunity
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DOI：
10.1016/j.vaccine.2003.10.007
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发表时间：
2004-03-29
期刊：
影响因子：
5.5
通讯作者：
Watanabe, H
中科院分区：
文献类型：
--
作者：
Koizumi, N;Watanabe, H
确定新的钩端螺旋体病候选疫苗是非常有意义的。本文对问号钩端螺旋体马尼拉血清型UP-MMC-NIID的两个同源免疫原蛋白LigA-m和LigB-m进行了鉴定，这两个蛋白与最近在问号钩端螺旋体波莫纳血清型中发现的LigA具有很强的相似性。Southern杂交分析表明，致病钩端螺旋体之间的lig基因是保守的。LIGA-m蛋白是体外培养过程中表达的一种表面暴露的脂蛋白。LigA-m和LigB-m均能诱导小鼠对问号钩端螺旋体同源血清型的攻击产生保护性免疫。此外，感染异种血清型的患者血清中含有抗lgA-m和ligB-m的抗体。我们的结果表明，LigA-m和LigB-m蛋白是预防钩端螺旋体病的新的候选疫苗。(C)2003爱思唯尔有限公司。保留所有权利。
The identification of new vaccine candidates for leptospirosis is of great interest. This paper describes the identification of two homologous immunogenic proteins, LigA-m and LigB-m, of Leptospira interrogans serovar manilae strain UP-MMC-NIID, which show strong similarity with LigA found recently in L. interrogans serovar pomona. Southern blot analysis indicated the conservation of the lig genes among pathogenic leptospires. LigA-m protein was shown to be a surface-exposed lipoprotein expressed during in vitro culture. Both LigA-m and LigB-m induced protective immunity against a challenge with a homologous serovar of L. interrogans in mice. Furthermore, patients' sera infected with heterologous serovars' strains contained antibodies against LigA-m and LigB-m. Our results suggest that LigA-m and LigB-m proteins are new vaccine candidates for the prevention of leptospirosis. (C) 2003 Elsevier Ltd. All rights reserved.