Identifying and characterizing Hfq-RNA interactions.
Identifying and characterizing Hfq-RNA interactions.
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DOI:
10.1016/j.ymeth.2013.04.023
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发表时间:
2013-09-15
期刊:
影响因子:
4.8
通讯作者:
Feig, A. L.
中科院分区:
文献类型:
--
作者:
Faner, M. A.;Feig, A. L.
To regulate stress responses and virulence, bacteria use small regulatory RNAs (sRNAs). These RNAs can up or down regulate target mRNAs through base pairing by influencing ribosomal access and RNA decay. A large class of these sRNAs, called trans-encoded sRNAs, requires the RNA binding protein Hfq to facilitate base pairing between the regulatory RNA and its target mRNA. The resulting network of regulation is best characterized in E. coli and S. typhimurium, but the importance of Hfq dependent sRNA regulation is recognized in a diverse population of bacteria. In this review we present the approaches and methods used to discover Hfq binding RNAs, characterize their interactions and elucidate their functions.
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