Phaeobacter gallaeciensis reduces Vibrio anguillarum in cultures of microalgae and rotifers, and prevents vibriosis in cod larvae.
Phaeobacter gallaeciensis reduces Vibrio anguillarum in cultures of microalgae and rotifers, and prevents vibriosis in cod larvae.
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DOI：
10.1371/journal.pone.0043996
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Gram L
中科院分区：
文献类型：
--
作者：
D'Alvise PW;Lillebø S;Prol-Garcia MJ;Wergeland HI;Nielsen KF;Bergh Ø;Gram L
无食腐杆菌具有体外拮抗鱼致病菌的作用，本研究的目的是评价该菌在海鱼幼鱼及其饲料培养中的应用价值。利用非拮抗突变体提出了拮抗益生菌的体内作用机制。gallaeciensis很容易在两种微藻水四藻（Tetraselmis suecica）和纳米绿藻（Nannochloropsis oculata）和轮虫（Brachionus plicatilis）的无菌培养中建立。gallaeciensis的密度为107 cfu/ml，对藻类和轮虫的生长没有不利影响。当引入这些培养物时，野生型P. gallaeciensis显著减少了鳗弧菌。一个不产生抗菌化合物tropodithic acid （TDA）的P. gallaeciensis突变体没有减少V. anguillarum的数量，这表明抗菌化合物的产生对P. gallaeciensis的拮抗特性很重要。采用每孔1只幼鱼的多培养皿系统，研究了gallaeciensis保护鱼幼鱼免受弧菌感染的能力。未攻毒幼虫的累计死亡率为40%，而攻毒后的累计死亡率为100%。P. gallaeciensis将攻毒鳕鱼幼虫（Gadus morhua）的死亡率降低至10%，显著低于攻毒和未攻毒幼虫的水平。TDA突变体降低了一些重复的鳕鱼幼虫的死亡率，尽管其程度远低于野生型。综上所示，gallaeciensis是一种很有前景的海洋幼体益生菌，TDA的生产可能是其益生作用的重要原因。
Phaeobacter gallaeciensis can antagonize fish-pathogenic bacteria in vitro, and the purpose of this study was to evaluate the organism as a probiont for marine fish larvae and their feed cultures. An in vivo mechanism of action of the antagonistic probiotic bacterium is suggested using a non-antagonistic mutant. P. gallaeciensis was readily established in axenic cultures of the two microalgae Tetraselmis suecica and Nannochloropsis oculata, and of the rotifer Brachionus plicatilis. P. gallaeciensis reached densities of 107 cfu/ml and did not adversely affect growth of algae or rotifers. Vibrio anguillarum was significantly reduced by wild-type P. gallaeciensis, when introduced into these cultures. A P. gallaeciensis mutant that did not produce the antibacterial compound tropodithietic acid (TDA) did not reduce V. anguillarum numbers, suggesting that production of the antibacterial compound is important for the antagonistic properties of P. gallaeciensis. The ability of P. gallaeciensis to protect fish larvae from vibriosis was determined in a bath challenge experiment using a multidish system with 1 larva per well. Unchallenged larvae reached 40% accumulated mortality which increased to 100% when infected with V. anguillarum. P. gallaeciensis reduced the mortality of challenged cod larvae (Gadus morhua) to 10%, significantly below the levels of both the challenged and the unchallenged larvae. The TDA mutant reduced mortality of the cod larvae in some of the replicates, although to a much lesser extent than the wild type. It is concluded that P. gallaeciensis is a promising probiont in marine larviculture and that TDA production likely contributes to its probiotic effect.
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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