Dose rate effect on micronuclei induction in human blood lymphocytes exposed to single pulse and multiple pulses of electrons
Dose rate effect on micronuclei induction in human blood lymphocytes exposed to single pulse and multiple pulses of electrons
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DOI：
10.1007/s00411-011-0353-1
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发表时间：
2011-05-01
影响因子：
1.7
通讯作者：
Narayana, Y.
中科院分区：
文献类型：
--
作者：
Acharya, Santhosh;Bhat, N. N.;Narayana, Y.
研究了7 MV电子单脉冲和多脉冲在宽剂量率（瞬时剂量率）范围内对人外周血淋巴细胞（PBL）微核（MN）诱导的影响。将PBL暴露于2、3、4、6和8戈伊的分级剂量的单电子脉冲，该单电子脉冲具有不同的脉冲宽度，每个脉冲的剂量率范围为1 x 10（6）戈伊s（-1）至3.2 x 10（8）戈伊s（-1）。通过调整束流和脉冲宽度来改变每个电子脉冲的剂量，从而获得不同的每个脉冲的剂量率。MN产率每单位吸收剂量照射后，与单电子脉冲的电子的多个脉冲进行了比较。MN产量的显着下降，随着每脉冲的剂量率的增加，观察到，当剂量是由一个单一的电子脉冲。然而，没有观察到MN产率的减少时，通过多个脉冲的电子传递剂量。在高剂量率每脉冲的产量下降表明可能的自由基复合，这导致减少生物损伤。细胞对大量染色体断裂的反应也可能改变损伤。
The effects of single pulses and multiple pulses of 7 MV electrons on micronuclei (MN) induction in cytokinesis-blocked human peripheral blood lymphocytes (PBLs) were investigated over a wide range of dose rates per pulse (instantaneous dose rate). PBLs were exposed to graded doses of 2, 3, 4, 6, and 8 Gy of single electron pulses of varying pulse widths at different dose rates per pulse, ranging from 1 x 10(6) Gy s(-1) to 3.2 x 10(8) Gy s(-1). Different dose rates per pulse were achieved by changing the dose per electron pulse by adjusting the beam current and pulse width. MN yields per unit absorbed dose after irradiation with single electron pulses were compared with those of multiple pulses of electrons. A significant decrease in the MN yield with increasing dose rates per pulse was observed, when dose was delivered by a single electron pulse. However, no reduction in the MN yield was observed when dose was delivered by multiple pulses of electrons. The decrease in the yield at high dose rates per pulse suggests possible radical recombination, which leads to decreased biological damage. Cellular response to the presence of very large numbers of chromosomal breaks may also alter the damage.