GWAS, QTL mapping and gene expression analyses in Brassica napus reveal genetic control of branching morphogenesis.
GWAS, QTL mapping and gene expression analyses in Brassica napus reveal genetic control of branching morphogenesis.
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甘蓝型油菜的 GWAS、QTL 作图和基因表达分析揭示了分枝形态发生的遗传控制
DOI：
10.1038/s41598-017-15976-4
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发表时间：
2017-11-21
影响因子：
4.6
通讯作者：
Qian W
中科院分区：
文献类型：
--
作者：
He Y;Wu D;Wei D;Fu Y;Cui Y;Dong H;Tan C;Qian W
油菜分支数是影响作物产量和品质的重要株型性状。在此，我们检测了DH群体及其重建的F2群体两年多的分支数量的QTL。此外，使用来自60 K Brassica Illumina® Infinium SNP阵列的具有33186个基因组SNP的一组327个种质进行关于分支数目的GWAS研究。通过连锁分析和关联作图相结合，将一个新的QTL精细定位到C 03上。随后，我们测试了SNP多态性与目标区间内基因的mRNA表达水平之间的相关性，以鉴定通过表达控制分支数量的潜在基因座或基因。结果表明，4个SNP位点与相应的基因表达水平相关，1个位点（BnaC 03 g63480 D）表现出表型变异与基因表达水平显著相关。我们的研究结果提供了深入了解分枝形态建成的遗传基础，并可能是有价值的优化架构在油菜育种。
Branch number is an important trait in plant architecture that can influence crop yield and quality in Brassica napus. Here, we detected the QTLs responsible for branch number in a DH population and its reconstructed F2 population over two years. Further, a GWAS research on branch number was performed using a panel of 327 accessions with 33186 genomic SNPs from the 60 K Brassica Illumina® Infinium SNP array. Through combining linkage analysis and association mapping, a new QTL was fine mapped onto C03. Subsequently, we tested the correlations between the SNP polymorphisms and mRNA expression levels of genes in the target interval to identify potential loci or genes that control branch number through expression. The results show that 4 SNP loci are associated with the corresponding gene expression levels, and one locus (BnaC03g63480D) exhibited a significant correlation between the phenotype variation and gene expression levels. Our results provide insights into the genetic basis for branching morphogenesis and may be valuable for optimizing architecture in rapeseed breeding.
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