Enhancement of immunity to an Escherichia coli vaccine in mice orally inoculated with a fusion gene encoding porcine interleukin 4 and 6
Enhancement of immunity to an Escherichia coli vaccine in mice orally inoculated with a fusion gene encoding porcine interleukin 4 and 6
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DOI：
10.1016/j.vaccine.2007.07.050
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发表时间：
2007-10-10
期刊：
影响因子：
5.5
通讯作者：
Gao, Rong
中科院分区：
文献类型：
--
作者：
Zhang, Huan;Cheng, Chi;Gao, Rong
本实验旨在研究壳聚糖纳米粒（CNPs）包裹猪IL-4/IL-6融合基因（PIL-4/IL-6）作为新型免疫佐剂的作用。构建IL 4/PIL 6融合基因，并将其插入真核表达载体。将该质粒与CNP结合，然后用于口服接种21日龄雌性昆明小鼠，同时肌肉注射大肠杆菌灭活疫苗。疫苗接种后35天，小鼠经口喂食强毒0 139：K88菌株EPEC E进行攻击。大肠杆菌与对照组相比，免疫球蛋白和特异性抗体的含量明显降低。结果表明，VPIL 4/IL 6-CNP免疫小鼠血清中的大肠杆菌数量显著增加（P < 0.05）。免疫小鼠血清中IL-2、IL-4和IL-6水平显著升高（P < 0.05）。攻击后，这些免疫学标志物在用融合基因构建体（IL 4/VPIL 6-CNP）或单独质粒（VPIL 4 + VPIL 6-CNP）免疫的小鼠中不同程度地升高。免疫小鼠在攻击后均存活，未显示任何症状或病变，而VR 1020-CNP对照小鼠表现出明显的临床症状和消化道出血性病变。这些结果表明，CNP包埋的VPIL 4/IL 6是一种新的有希望的佐剂，以促进动物对感染性病原体的特异性免疫和抵抗。(c)2007爱思唯尔有限公司保留所有权利。
Experiments were conducted to investigate the effect of a fusion gene of porcine IL-4 and IL-6 (PIL4/IL6) packaged with chitosan nanoparticles (CNPs) in terms of the development of a novel effective adjuvant. The IL4/PIL6 fusion gene was constructed and inserted into a eukaryotic expression vector. The plasmid was bound to CNP and then utilized to orally inoculate 21-day-old female Kunming mice that simultaneously received intramuscular injection of inactivated Escherichia coli vaccine. At 35 days post-vaccination, the mice were challenged by oral feeding with virulent 0 139: K88 strain EPEC E. coli bacteria. Compared with those of control mice, the content of immunoglobulins and specific antibodies to E. coli increased significantly in the sera of mice immunized with VPIL4/IL6-CNP (P < 0.05). Furthermore, the levels of IL-2, IL-4 and IL-6 increased remarkably in the sera of immunized mice (P < 0.05). After challenge, these immunological markers were elevated to different degrees in the mice immunized with the fusion gene construct (lL4/VPlL6-CNP) or individual plasmids (VPIL4 + VPIL6-CNP). The immunized mice all survived the challenge and did not show any symptoms or lesion, whereas the VR 1020-CNP control mice manifested obvious clinical symptoms and hemorrhagic lesions in the digestive tracts. These results demonstrated that VPIL4/IL6 entrapped with CNP is a novel promising adjuvant to promote specific immunity and resistance of animals against infectious pathogen. (c) 2007 Elsevier Ltd. All rights reserved.