EFFECT OF PROTEIN DEGRADATION ON CELLULAR GROWTH-CHARACTERISTICS
EFFECT OF PROTEIN DEGRADATION ON CELLULAR GROWTH-CHARACTERISTICS
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DOI：
10.1002/jcp.1040960202
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发表时间：
1978-01-01
影响因子：
5.6
通讯作者：
STANNERS, CP
中科院分区：
文献类型：
--
作者：
BAXTER, GC;STANNERS, CP
通过测定3种细胞在不同生长条件下不稳定蛋白和稳定蛋白的降解率，研究了蛋白质降解在细胞增殖中的作用。蛋白质的降解率是一个相对不变的参数，因为在环己亚胺或组氨酸醇对蛋白质合成的强烈抑制后，蛋白质的降解率并没有改变。在指数期和平稳期均相同，与恶性转化的存在与否无关。使用3种分裂率差别很大的不同细胞类型[仓鼠、大鼠和多瘤病毒转化的仓鼠无性系]，细胞分裂率和DNA合成率（以%/h计）正好等于蛋白质积累率（以%/h计），即蛋白质合成率减去蛋白质降解率。不同细胞类型之间的分裂速率主要由蛋白质合成速率决定，但在低分裂速率下，蛋白质降解速率可以代表蛋白质积累速率的很大一部分。
The role of protein degradation in cellular proliferation was investigated by measurements of the rates of degradation of labile and stable proteins for 3 cell types under various growth conditions. The rate of protein degradation is a relatively invariant parameter as it did not change after strong inhibition of protein synthesis with cycloheximide or histidinol. It was the same in both exponential and stationary phase, and it did not correlate with the presence or absence of malignant transformation. Using 3 different cell types [hamster, rat, and clonal lines of hamster transformed by polyoma virus] with widely differing division rates, the rate of cell division and DNA synthesis (in %/h) is precisely equal to the rate of protein accumulation (in %/h), i.e., to the rate of protein synthesis minus the rate of protein degradation. Division rates between the different cell types were determined chiefly by the rate of protein synthesis but at low division rates, the rate of protein degradation could represent a large component of the protein accumulation rate.