Serotype Is Associated With High Rate of Colistin Resistance Among Clinical Isolates of Salmonella.
Serotype Is Associated With High Rate of Colistin Resistance Among Clinical Isolates of Salmonella.
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沙门氏菌临床分离株中血清型与高粘菌素耐药率相关
DOI：
10.3389/fmicb.2020.592146
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发表时间：
2020
影响因子：
5.2
通讯作者：
Xiao Y
中科院分区：
文献类型：
--
作者：
Luo Q;Wang Y;Fu H;Yu X;Zheng B;Chen Y;Berglund B;Xiao Y
为了解我国临床分离的沙门氏菌对粘菌素的耐药情况、耐药机制及血清型相关性，从患者粪便和血液中分离沙门氏菌。本研究中，42.8%（136/318）的临床分离沙门氏菌对粘菌素耐药。在中国人群中最流行的两种血清型中，肠杆菌对粘菌素的MIC分布（83.9%耐药，125/149）明显低于鼠伤寒沙门氏菌（15.3%耐药，9/59）（P < 0.01）。PmrAB中的mcr基因和突变对从人类患者中分离的粘菌素耐药沙门氏菌的发生率影响很小。基于核心基因组单核苷酸多态性（SNPs）构建的系统发育树按血清型分开，提示MIC在同一血清型分离株中呈扩散分布。在非mcr粘菌素耐药的S.鼠伤寒沙门氏菌比粘菌素敏感的S。鼠伤寒沙门氏菌，但没有可辨别的差异之间的粘菌素耐药和敏感的S。肠内感染表明，粘菌素耐药的S。鼠伤寒沙门氏菌（S.肠道。总之，临床分离沙门氏菌的粘菌素敏感性和粘菌素耐药机制与特定的血清型密切相关，至少在两个最流行的血清型肠杆菌和鼠伤寒沙门氏菌。我们建议临床微生物实验室在血清型水平解释沙门氏菌粘菌素MIC结果。
To investigate the prevalence, probable mechanisms and serotype correlation of colistin resistance in clinical isolates of Salmonella from patients in China, Salmonella isolates were collected from fecal and blood samples of patients. In this study, 42.8% (136/318) clinical isolated Salmonella were resistant to colistin. MIC distribution for colistin at serotype level among the two most prevalent serotypes originating from humans in China indicated that Salmonella Enteritidis (83.9% resistance, 125/149) were significantly less susceptible than Salmonella Typhimurium (15.3% resistance, 9/59, P < 0.01). mcr genes and mutations in PmrAB confer little for rate of colistin resistant Salmonella isolated from human patients. Phylogenetic tree based on core-genome single nucleotide polymorphisms (SNPs) was separately by the serotypes and implied a diffused distribution of MICs in the same serotype isolates. Relatvie expression levels of colistin resistant related pmr genes were significantly higher in non-mcr colistin resistant S. Typhimurium than in colistin sensitive S. Typhimurium, but no discernable differences between colistin resistant and sensitive S. Enteritidis, indicating a different mechanism between colistin resistant S. Typhimurium and S. Enteritidis. In conclusion, colistin susceptibility and colistin resistant mechanism of clinical isolated Salmonella were closely associated with specific serotypes, at least in the two most prevalent serotype Enteritidis and Typhimurium. We suggest clinical microbiology laboratory interpreting Salmonella colistin MIC results in the serotype level.
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影响因子：
5.8
作者：
Gardner, Shea N.;Slezak, Tom;Hall, Barry G.
通讯作者：
Hall, Barry G.
影响因子：
5.2
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通讯作者：
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影响因子：
14.9
作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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