Distribution of bone morphogenetic protein and bone morphogenetic protein receptor transcripts in the rodent nervous system and up-regulation of bone morphogenetic protein receptor type II in hippocampal dentate gyrus in a rat model of global cerebral ischemia
Distribution of bone morphogenetic protein and bone morphogenetic protein receptor transcripts in the rodent nervous system and up-regulation of bone morphogenetic protein receptor type II in hippocampal dentate gyrus in a rat model of global cerebral ischemia
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DOI：
10.1016/s0306-4522(00)00246-3
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发表时间：
2000-01-01
期刊：
影响因子：
3.3
通讯作者：
Ruat, M
中科院分区：
文献类型：
--
作者：
Charytoniuk, DA;Traiffort, E;Ruat, M
骨形态发生蛋白属于转化生长因子-p超家族，通过丝氨酸/苏氨酸激酶I型和II型受体起作用，如骨形态发生蛋白受体I型和II型。为了进一步了解这些因子在神经系统中的作用，我们检测了7种骨形态发生蛋白和骨形态发生蛋白受体I型和II型转录本在啮齿动物脑和脊髓中的表达模式。尽管骨形态发生蛋白受体I型在大鼠脑中的表达较低，但使用特异性地高辛标记的核糖核酸探针进行的原位杂交研究显示，整个大脑中存在骨形态发生蛋白受体ii型阳性细胞，其中在黑质多巴胺能细胞中有明显的定位。位于脊髓前角的大运动神经元样细胞和背根神经节的感觉神经元也表达骨形态发生蛋白受体Il型转录本。此外，我们观察到大鼠短暂性全脑缺血48小时后齿状体颗粒细胞中骨形态发生蛋白受体II型的显著上调，这表明该受体的调节干预了脑损伤后发生的神经元可塑性或修复。在该受体的潜在配体中，骨形态发生蛋白-6和骨形态发生蛋白-7在脑膜和脉络膜丛中表达，而表达骨形态发生蛋白-4的细胞在发育和成年期间在有髓鞘结构中受到空间和时间的调节，表明其在少突胶质细胞中表达。这些数据清楚地表明，骨形态发生蛋白及其受体除了在骨和胚胎组织中发挥作用外，还可能在成人神经组织中发挥重要作用。(c) 2000 ibro。Elsevier Science Ltd.出版。版权所有。
Bone morphogenetic proteins belong to the transforming growth factor-p superfamily and act through serine/threonine kinase type I and type II receptors such as bone morphogenetic protein receptor type I and type II. In order to further understand the roles that these factors exert in the nervous system, we have examined the expression pattern of seven bone morphogenetic proteins and bone morphogenetic protein receptor type I and II transcripts in the brain and spinal cord of rodent. Whereas bone morphogenetic protein receptor type I expression was low in rat brain, in situ hybridization studies performed with specific digoxigenin-labelled riboprobes revealed the presence of bone morphogenetic protein receptor type II-positive cells throughout the brain, with a notable localization in dopaminergic cells of the substantia nigra. Bone morphogenetic protein receptor type Il transcripts were also expressed by large motoneuron-like cells located in the ventral horn of the spinal cord and by sensory neurons of dorsal root ganglia. In addition, we observed a significant up-regulation of bone morphogenetic protein receptor type II in the granule cells of the dentate gyms 48 h after transient global cerebral ischemia in rat suggesting that modulation of this receptor intervenes during neuronal plasticity or repair that occur upon brain injury. Among the potential ligands for this receptor, bone morphogenetic protein-6 and bone morphogenetic protein-7 were expressed in meninges and the choroid plexus, while bone morphogenetic protein-4-expressing cells were spatially and temporally regulated in myelinated structures during development and in the adult suggesting its expression in oligodendrocytes.These data clearly indicate that besides their roles in bone and embryonic tissues, bone morphogenetic proteins and their receptors may have also important functions in adult neural tissues. (C) 2000 IBRO. Published by Elsevier Science Ltd. All rights reserved.