Ethanol production from SPORL-pretreated lodgepole pine: preliminary evaluation of mass balance and process energy efficiency
Ethanol production from SPORL-pretreated lodgepole pine: preliminary evaluation of mass balance and process energy efficiency
复制标题
DOI：
10.1007/s00253-009-2408-7
复制
发表时间：
2010-05-01
影响因子：
5
通讯作者：
Pan, X. J.
中科院分区：
文献类型：
--
作者：
Zhu, J. Y.;Zhu, Wenyuan;Pan, X. J.
来自森林间伐的黑松是一种潜在的乙醇生产原料。在这项研究中，黑松转化为乙醇的产量为276升每公吨木材或72%的理论产量。为克服木质纤维素（SPORL）预处理的难降解性，将松木屑直接进行亚硫酸盐预处理，然后盘磨，回收的纤维素底物采用商品纤维素酶和酿酒酵母D5 A进行半同步酶法糖化和发酵生产乙醇。来自预处理的含有主要来自半纤维素的水解糖的液体流在使用XAD树脂柱脱毒后通过相同的酵母菌株发酵。SPORL预处理在实验室蒸煮器中以3：1（v/w）的液木比在180 A摄氏度下进行25分钟的时间。采用三种水平的硫酸进料（基于烘干木材，以w/w计为0.0%、1.4%和2.2%）和三种水平的亚硫酸氢钠进料（以w/w计为0.0%、4.0%和8.0%）。测定了研磨和预处理的机械能耗和热能消耗。这些数据用于确定糖回收率和净乙醇能量生产值的效率，并制定初步的质量和能量平衡。
Lodgepole pine from forest thinnings is a potential feedstock for ethanol production. In this study, lodgepole pine was converted to ethanol with a yield of 276 L per metric ton of wood or 72% of theoretical yield. The lodgepole pine chips were directly subjected to sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) pretreatment and then disk-milled; the recovered cellulose substrate was quais-simultaneously saccharified enzymatically and fermented to ethanol using commercial cellulases and Saccharomyces cerevisiae D5A. The liquor stream from the pretreatment containing hydrolyzed sugars mainly from hemicelluloses was fermented by the same yeast strain after detoxification using an XAD resin column. The SPORL pretreatment was conducted at 180A degrees C for a period of 25 min with a liquor-to-wood ratio of 3:1 (v/w) in a laboratory digester. Three levels of sulfuric acid charge (0.0%, 1.4%, and 2.2% on an oven dried wood basis in w/w) and three levels of sodium bisulfite charge (0.0%, 4.0%, and 8.0% in w/w) were applied. Mechanical and thermal energy consumption for milling and pretreatment were determined. These data were used to determine the efficiency of sugar recoveries and net ethanol energy production values and to formulate a preliminary mass and energy balance.