Human PinX1, a potent telomerase inhibitor, is not involved in human gastrointestinal tract carcinoma.
Human PinX1, a potent telomerase inhibitor, is not involved in human gastrointestinal tract carcinoma.
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DOI：
10.3892/or.11.4.871
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发表时间：
2004-04
期刊：
影响因子：
4.2
通讯作者：
Y. Akiyama;C. Maesawa;K. Wada;K. Fujisawa;T. Itabashi;Yoshinori Noda;T. Honda;N. Sato;K. Ishid
中科院分区：
文献类型：
--
作者：
Y. Akiyama;C. Maesawa;K. Wada;K. Fujisawa;T. Itabashi;Yoshinori Noda;T. Honda;N. Sato;K. Ishid
PinX1是一种Pin2/TRF1结合蛋白，也与端粒酶催化亚单位hTERT结合。该基因是一种有效的端粒酶抑制因子，也是一种假定的肿瘤抑制因子，因为它能抑制端粒酶活性并影响裸鼠体内的致瘤性。本研究旨在探讨胃肠道肿瘤(GITCs)中PinX1基因的突变情况。我们检测了15个GITC细胞系和20例原发GITC患者的PinX1基因突变、mRNA表达和启动子甲基化。我们在一个结肠癌和一个食道癌细胞系中发现了AGC/TGC错义突变，在2例原发GITC患者的癌组织和匹配的正常组织中也发现了AGC/TGC错义突变(10%)。这可能是一种良性的多态。5-氮-2‘-脱氧胞苷处理不影响任何细胞株PinX1基因的表达水平。结论：人PinX1基因不影响人GITC的致瘤作用。
PinX1 was isolated as a Pin2/TRF1 binding protein that also binds to the telomerase catalytic subunit hTERT. The gene is a potent telomerase inhibitor and a putative tumor suppressor since it inhibits telomerase activity and affects tumorigenicity in nude mice. This study investigated aberrations of PinX1 gene in gastrointestinal tract carcinomas (GITCs). We examined mutations, mRNA expression and promoter methylation of PinX1 gene in 15 GITC cell lines, and 20 patients with primary GITC. We found a missense mutation at codon 254 (AGC/TGC) in a colon and an esophageal carcinoma cell line, and in cancerous and matching normal tissues of 2 patients with primary GITC (10%). It might be a benign polymorphism. No hyper-methylation was found in the promoter region and the treatment by 5-Aza-2'-deoxycytidine did not affect PinX1 mRNA expression level in any of the cell lines. It was concluded that the human PinX1 does not affect tumorigenesis of human GITC.