Leptospira species molecular epidemiology in the genomic era
Leptospira species molecular epidemiology in the genomic era
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DOI：
10.1016/j.meegid.2017.08.013
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发表时间：
2017-10-01
影响因子：
3.2
通讯作者：
Ruybal, P.
中科院分区：
文献类型：
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作者：
Caimi, K.;Repetto, S. A.;Ruybal, P.
钩端螺旋体病是一种人畜共患疾病，其全球负担日益增加，往往与气候变化有关。来自世界各地钩端螺旋体属分离株的数百个全基因组序列。现在，连同允许直接从原始序列数据分配MLST序列类型（ST）的在线工具一起可用。在这项工作中，我们将R7 L-MLST应用于全球分布的近500个基因组和菌株收集。所有10种致病菌种以及中间体均使用该MLST方案进行分型。在我们以前的工作中观察到的ST和血清群之间的相关性仍然满足于这个更高的数据集，支持实施MLST，以辅助血清学分类作为一种补充方法。从R7-MLST位点的串联序列的贝叶斯系统发育分析，使我们能够解决分类学上的不一致，但也表明，事件，如重组，基因转换或横向基因转移钩端螺旋体属的进化中发挥了重要作用。全基因组测序使我们能够提供适当的流行病学信息，这些信息有助于设计控制策略和诊断方法。
Leptospirosis is a zoonotic disease which global burden is increasing often related to climatic change. Hundreds of whole genome sequences from worldwide isolates of Leptospira spp. are available nowadays, together with online tools that permit to assign MLST sequence types (STs) directly from raw sequence data. In this work we have applied R7L-MLST to near 500 genomes and strains collection globally distributed. All 10 pathogenic species as well as intermediate were typed using this MLST scheme. The correlation observed between STs and serogroups in our previous work, is still satisfied with this higher dataset sustaining the implementation of MLST to assist serological classification as a complementary approach. Bayesian phylogenetic analysis of concatenated sequences from R7-MLST loci allowed us to resolve taxonomic inconsistencies but also showed that events such as recombination, gene conversion or lateral gene transfer played an important role in the evolution of Leptospira genus. Whole genome sequencing allows us to contribute with suitable epidemiologic information useful to apply in the design of control strategies and also in diagnostic methods for this illness.