Analysis by DC-EPG of the resistance to Bemisia tabaci on an Mi-tomato line
Analysis by DC-EPG of the resistance to Bemisia tabaci on an Mi-tomato line
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DOI：
10.1023/a:1019240418595
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发表时间：
2001-01-01
影响因子：
1.9
通讯作者：
Muñiz, M
中科院分区：
文献类型：
--
作者：
Jiang, YX;Nombela, G;Muñiz, M
番茄Mi基因赋予对线虫，根结线虫属，以及马铃薯蚜虫，巨管蚜（Macrosiphum euphorphum（托马斯））。以前的温室选择测定与烟粉虱（Gennadius）表明，携带该基因的番茄商业品种有显着较低的值的主机适合性和粉虱繁殖比品种缺乏Mi。这表明Mi或其区域的另一个基因可以调节部分抗性。为了验证B型烟粉虱的这种抗性，采用直流电穿透图（EPG）技术研究了B型烟粉虱在番茄近等基因系Moneymaker（不含Mi）和Motelle（携带Mi）上的探测和取食行为。番茄品系间表皮和叶肉组织EPG参数差异显著（P < 0.05）。在Motelle上，较低百分比的粉虱达到韧皮部阶段，并且它们在达到第一韧皮部阶段之前制造更多的探针，具有较高的比率（第一韧皮部阶段之前的探针数目）/（探针总数），具有较长的非探测时间的总持续时间，以及在进行第一次细胞内穿刺之前和在进行第一韧皮部阶段之前的较长时间。与此相反，大多数参数相关的韧皮部阶段被发现没有显着差异，这些近等基因系。的行为数据强烈表明，在品种Motelle的部分电阻是由于在表皮和/或叶肉的因素，抑制粉虱达到韧皮部筛元素。然而，一旦管芯到达筛元件，两个品种之间的粉虱行为就没有什么不同。因此，这两个品种的韧皮部汁液对粉虱来说似乎是同样可接受的。进一步的研究是必要的，以提供更好地了解这些机制的抗粉虱在番茄。
The tomato Mi gene confers resistance to nematodes, Meloidogyne spp., and to the potato aphid, Macrosiphum euphorbiae (Thomas). Previous greenhouse choice assays with Bemisia tabaci (Gennadius) showed that tomato commercial varieties carrying this gene had significantly lower values of host suitability and whitefly reproduction than varieties lacking Mi. This indicated that Mi, or another gene in its region, could regulate partial resistance. In order to characterise this resistance, probing and feeding behaviour of Bemisia tabaci B-biotype was studied with DC Electrical Penetration Graph (EPG) technique on the near-isogenic tomato lines Moneymaker (without Mi) and Motelle (carrying Mi). Significant differences (P < 0.05) between tomato lines were found in EPG parameters related to epidermis and/or mesophyll tissues. On Motelle, a lower percentage of whiteflies achieved phloem phase and they made more probes before attaining first phloem phase, had a higher ratio (number of probes before first phloem phase)/(total number of probes), had a longer total duration of non-probing time, and a longer time before making the first intracellular puncture and before making the first phloem phase. In contrast, most of the parameters related to phloem phase were found not to differ significantly between these near-isogenic lines. The behavioural data strongly suggest that the partial resistance in the variety Motelle is due to factors in the epidermis and/or mesophyll that inhibit the whiteflies from reaching phloem sieve elements. However, once the stylets reach a sieve element, whitefly behaviour did not differ between the two varieties. Thus, phloem sap of the two varieties appears to be equally acceptable to the whiteflies. Further studies are necessary to provide a better understanding of these mechanisms of resistance to whiteflies in tomatoes.