Color-Coded Super-Resolution Small-Molecule Imaging.
Color-Coded Super-Resolution Small-Molecule Imaging.
复制标题
颜色编码的超分辨率小分子成像。
DOI：
10.1002/cbic.201600013
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Cang,Hu
中科院分区：
文献类型：
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作者：
Beuzer,Paolo;LaClair,JamesJ;Cang,Hu
虽然超分辨率显微镜的发展可以追溯到1994年，但其应用主要集中在可视化细胞结构和目标，包括蛋白质，DNA和糖。我们现在报告一个系统，该系统允许在低分辨率下监测活细胞中外源小分子的定位，并随后通过使用固定细胞上的随机光学重建显微镜（STORM）进行超分辨率成像。这是一个强大的新工具，了解与外源性小分子的作用模式和机制相关的亚细胞运输的动态。
Although the development of super‐resolution microscopy dates back to 1994, its applications have been primarily focused on visualizing cellular structures and targets, including proteins, DNA and sugars. We now report on a system that allows both monitoring of the localization of exogenous small molecules in live cells at low resolution and subsequent super‐resolution imaging by using stochastic optical reconstruction microscopy (STORM) on fixed cells. This represents a powerful new tool to understand the dynamics of subcellular trafficking associated with the mode and mechanism of action of exogenous small molecules.