Desulfomonile limimaris sp. nov., an anaerobic dehalogenating bacterium from marine sediments.
Desulfomonile limimaris sp. nov., an anaerobic dehalogenating bacterium from marine sediments.
复制标题
脱磺菌 limimaris sp.
DOI：
--
复制
发表时间：
2001
影响因子：
2.8
通讯作者：
J. Tiedje
中科院分区：
文献类型：
--
作者：
Baolin Sun;J. Cole;J. Tiedje
菌株DCB-MT和DCB-F是从海洋沉积物中富集的厌氧3-氯苯甲酸盐（3CB）矿化培养物中分离得到的。分离物是大的革兰氏阴性杆菌，每个细胞都有一个环。分离株是专性厌氧菌，能够将3CB还原脱氯为苯甲酸盐。在菌株DCB-MT中通过氯呼吸生长产生1.7 g蛋白质mol（-1）3CB，用乳酸盐作为电子供体脱氯。菌株DCB-MT也能利用富马酸盐、硫酸盐、亚硫酸盐、硫代硫酸盐和硝酸盐作为生理电子受体生长，但在硫酸盐和硝酸盐上生长较差。亚硫酸盐和硫代硫酸盐完全抑制还原脱氯，但硫酸盐不抑制。两种菌株在NaCl浓度低于0.32%（w/v）时均不能生长。他们生长良好，在海水中的盐浓度，然而，最佳的增长速度是在NaCl浓度的一半，海水中实现。16 S rDNA序列分析显示菌株DCB-MT和DCB-F彼此相似性为99%，并且与它们最近的亲戚，脱硫菌菌株DCB-1 T相似性为93%。菌株DCB-MT与菌株DCB-1 T的区别还在于其不能使用乙酸盐在3CB上生长，并且需要NaCl。DCB-MT和DCB-F的形态、生理和16 S rDNA序列表明，这些菌株代表了一个新的海洋适应性物种，命名为Desulfomonile limimaris sp. nov.。模式菌株是菌株DCB-MT（= ATCC 700979 T）。
Strains DCB-MT and DCB-F were isolated from anaerobic 3-chlorobenzoate (3CB)-mineralizing cultures enriched from marine sediments. The isolates are large, Gram-negative rods with a collar girdling each cell. The isolates are obligate anaerobes capable of reductive dechlorination of 3CB to benzoate. Growth by chlororespiration in strain DCB-MT yielded 1.7 g protein mol(-1) 3CB dechlorinated with lactate as the electron donor. Strain DCB-MT also used fumarate, sulfate, sulfite, thiosulfate and nitrate as physiological electron acceptors for growth, but grew poorly on sulfate and nitrate. Reductive dechlorination was inhibited completely by sulfite and thiosulfate but not by sulfate. Both strains were incapable of growth at NaCl concentrations below 0.32% (w/v). They grew well at sea-water salt concentrations; however, the optimum growth rate was achieved at a NaCl concentration half that of sea water. The 16S rDNA sequence analysis shows strains DCB-MT and DCB-F to be 99% similar to each other and 93% similar to their closest relative, Desulfomonile tiedjei strain DCB-1T. Strain DCB-MT can also be distinguished from strain DCB-1T by its inability to use acetate for growth on 3CB and by its requirement for NaCl. The morphology, physiology and 16S rDNA sequences of DCB-MT and DCB-F suggest that these strains represent a new, marine-adapted species of the genus Desulfomonile, designated Desulfomonile limimaris sp. nov. The type strain is strain DCB-MT (= ATCC 700979T).