UPTAKE OF ORALLY-ADMINISTERED BETA-CAROTENE BY BLOOD-PLASMA, LEUKOCYTES, AND LIPOPROTEINS IN CALVES
UPTAKE OF ORALLY-ADMINISTERED BETA-CAROTENE BY BLOOD-PLASMA, LEUKOCYTES, AND LIPOPROTEINS IN CALVES
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DOI：
10.2527/1993.713730x
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发表时间：
1993-03-01
影响因子：
3.3
通讯作者：
MICHAL, JJ
中科院分区：
文献类型：
--
作者：
CHEW, BP;WONG, TS;MICHAL, JJ
研究了口服单一剂量(Exp.1；n=18头安格斯小牛)或多头(实验2；n=16头荷斯坦犊牛)剂量的β-胡萝卜素。服用β-胡萝卜素会增加血浆中的β-胡萝卜素和与每个脂蛋白组分相关的β-胡萝卜素的量。在β-胡萝卜素治疗之前，与高密度脂蛋白(HDL)相关的β-胡萝卜素总量比与低密度脂蛋白(LDL)相关的数量高出三到四倍，比与极低密度脂蛋白(VLDL)相关的数量高出五倍。与脂蛋白相关的总β-胡萝卜素在低密度脂蛋白中的相对增加大于高密度脂蛋白或极低密度脂蛋白。口服β-胡萝卜素可增加淋巴细胞对β-胡萝卜素的摄取。从喂食β-胡萝卜素的动物的血液淋巴细胞中分离出的亚细胞部分显示，线粒体、核和微体部分大量摄取了β-胡萝卜素。这些亚细胞组分摄取β-胡萝卜素的情况与在血浆中观察到的情况并不一致。相比之下，无论添加或不添加β-胡萝卜素，都不能在血液中性粒细胞和红细胞中检测到β-胡萝卜素。治疗不影响血浆、脂蛋白、淋巴细胞、中性粒细胞或红细胞中视黄醇或α-生育酚的浓度。这些数据揭示了牛淋巴细胞亚细胞组分中β-胡萝卜素的存在，并暗示了β-胡萝卜素在这些细胞中可能的生理作用。
The uptake of beta-carotene by blood cells, plasma, and lipoproteins was studied in bull calves that were orally administered a single (Exp. 1; n = 18 Angus calves) or multiple (Exp. 2; n = 16 Holstein calves) doses of beta-carotene. Administration of beta-carotene increased plasma beta-carotene and the amount of beta-carotene associated with each lipoprotein fraction. Before beta-carotene treatment, the total amount of beta-carotene associated with the high-density lipoprotein (HDL) was three- to fourfold higher than the amount associated with low-density lipoprotein (LDL) and fivefold higher than the amount associated with very-low-density lipoprotein (VLDL). The relative increase in total beta-carotene associated with the lipoproteins was greater for LDL than for HDL or VLDL. Orally administered beta-carotene increased the uptake of beta-carotene by lymphocytes. Subcellular fractions of blood lymphocytes isolated from animals fed beta-carotene revealed that beta-carotene was taken up in significant amounts by the mitochondrial, nuclear, and microsomal fractions. The profile of beta-carotene uptake by these subcellular fractions did not mirror that observed in plasma. In contrast, beta-carotene was not detectable in blood neutrophils and erythrocytes in either beta-carotene-supplemented or unsupplemented calves. Treatment did not influence the concentrations of retinol or alpha-tocopherol in plasma, lipoproteins, lymphocytes, neutrophils, or erythrocytes. These data revealed the presence of beta-carotene in bovine lymphocyte subcellular fractions and suggest a possible physiological role of beta-carotene in these cells.