Connexin36 mediates spike synchrony in olfactory bulb glomeruli
Connexin36 mediates spike synchrony in olfactory bulb glomeruli
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DOI：
10.1016/j.neuron.2005.04.030
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发表时间：
2005-06-02
期刊：
影响因子：
16.2
通讯作者：
Westbrook, GL
中科院分区：
文献类型：
--
作者：
Christie, JM;Bark, C;Westbrook, GL
神经元同步对于许多大脑区域的网络行为很重要。在嗅球中，主要神经元（二尖瓣细胞）将顶端树突投射到共同的肾小球，在那里它们接收共同的输入。肾小球内的同步活动取决于化学传递，但二尖瓣细胞也是电耦合的。我们研究了连接蛋白介导的间隙连接在二尖瓣细胞协调活动中的作用。在 connexin36 (Cx36) 敲除小鼠中，投射到同一肾小球的二尖瓣细胞之间的电耦合和相关尖峰完全不存在。肾小球的超微结构分析证实远端顶端树突上的二尖瓣-二尖瓣细胞间隙连接含有 Cx36。在敲除过程中，二尖瓣细胞对之间的耦合 AMPA 反应不存在，这表明是电耦合而不是递质溢出负责同步。我们的结果表明，二尖瓣细胞之间的 Cx36 介导的间隙连接通过一种新型电化学传输形式协调快速协调信号传导。
Neuronal synchrony is important to network behavior in many brain regions. In the olfactory bulb, principal neurons (mitral cells) project apical dendrites to a common glomerulus where they receive a common input. Synchronized activity within a glomerulus depends on chemical transmission but mitral cells are also electrically coupled. We examined the role of connexin-mediated gap junctions in mitral cell coordinated activity. Electrical coupling as well as correlated spiking between mitral cells projecting to the same glomerulus was entirely absent in connexin36 (Cx36) knockout mice. Ultrastructural analysis of glomeruli confirmed that mitral-mitral cell gap junctions on distal apical dendrites contain Cx36. Coupled AMPA responses between mitral cell pairs were absent in the knockout, demonstrating that electrical coupling, not transmitter spillover, is responsible for synchronization. Our results indicate that Cx36-mediated gap junctions between mitral cells orchestrate rapid coordinated signaling via a novel form of electrochemical transmission.