A whole blood thrombus mimic: Constitutive behavior under simple shear
A whole blood thrombus mimic: Constitutive behavior under simple shear
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DOI：
10.1016/j.jmbbm.2020.104216
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发表时间：
2021-01-21
影响因子：
3.9
通讯作者：
Rausch, Manuel K.
中科院分区：
文献类型：
--
作者：
Sugerman, Gabriella P.;Kakaletsis, Sotirios;Rausch, Manuel K.
在美国，深静脉血栓和肺栓塞每年影响 300,000-600,000 名患者。为了更好地了解肺栓塞的高度机械病理生理学，我们着手开发一种体外血栓模拟物，并在大变形简单剪切下测试这种模拟物。除了报告我们的模拟物在简单剪切下的力学之外，我们还探索了它们的力学对凝血条件和血液储存时间的敏感性，并比较了三种超弹性材料模型的拟合我们数据的能力。我们发现，当在简单剪切下进行 50% 应变的准静态测试时，由全血制成的血栓模拟物表现出应变硬化、负坡印廷效应和滞后现象。此外，我们发现这些模拟物的硬度不会随凝血条件或血液储存时间而显着变化。在我们测试的三个超弹性本构模型中，Ogden 模型提供了对剪切应力和正应力的最佳拟合。总之，我们开发了一种强大的协议来生成规则形状、均匀的血栓模拟物，使其能够在大变形下进行简单的剪切测试。未来的研究将扩展我们的模型以包括成熟的影响并探索其断裂特性，以更好地理解栓塞。
Deep vein thrombosis and pulmonary embolism affect 300,000-600,000 patients each year in the US. To better understand the highly mechanical pathophysiology of pulmonary embolism, we set out to develop an in-vitro thrombus mimic and to test this mimic under large deformation simple shear. In addition to reporting on the mechanics of our mimics under simple shear, we explore the sensitivity of their mechanics to coagulation conditions and blood storage time, and compare three hyperelastic material models for their ability to fit our data. We found that thrombus mimics made from whole blood demonstrate strain-stiffening, a negative Poynting effect, and hysteresis when tested quasi-statically to 50% strain under simple shear. Additionally, we found that the stiffness of these mimics does not significantly vary with coagulation conditions or blood storage times. Of the three hyperelastic constitutive models that we tested, the Ogden model provided the best fits to both shear stress and normal stress. In conclusion, we developed a robust protocol to generate regularly-shaped, homogeneous thrombus mimics that lend themselves to simple shear testing under large deformation. Future studies will extend our model to include the effect of maturation and explore its fracture properties toward a better understanding of embolization.