Development of ligament-like structural organization and properties in cell-seeded collagen scaffolds in vitro
Development of ligament-like structural organization and properties in cell-seeded collagen scaffolds in vitro
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DOI：
10.1007/s10439-005-9058-4
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发表时间：
2006-05-01
影响因子：
3.8
通讯作者：
Nauman, Eric A.
中科院分区：
文献类型：
--
作者：
Gentleman, Eileen;Livesay, Glen A.;Nauman, Eric A.
急性前交叉韧带（ACL）损伤导致关节功能差、不稳定，如果不及时治疗，最终会导致骨关节炎。目前的手术治疗方案并不理想;然而，组织工程可以提供机械上可靠的、生物相容的重建。将胶原纤维支架与成纤维细胞接种的胶原凝胶组合，并在培养物中维持长达20天。结构的拉伸和粘弹性行为与天然韧带的拉伸和粘弹性行为非常相似。在培养中，结构的机械和粘弹性不会随着时间的推移而降低，并且具有包埋成纤维细胞的结构的峰值应力显著更高。免疫细胞化学和组织学分析表明细胞增殖和韧带样组织。我们已经创建了一种工程组织，其接近ACL的关键机械和粘弹性特性，在培养20天后不会降解，并且在组织学上与天然组织相似。这项研究将有助于开发有效的治疗ACL损伤。
Acute anterior cruciate ligament (ACL) injuries lead to poor joint function, instability, and eventually osteoarthritis if left untreated. Current surgical treatment options are not ideal; however, tissue engineering may provide mechanically sound, biocompatible reconstructions. Collagen fiber scaffolds were combined with fibroblast-seeded collagen gels and maintained in culture for up to 20 days. The tensile and viscoelastic behavior of the constructs closely mimicked that of natural ligament. Constructs' mechanical and viscoelastic properties did not degrade over time in culture, and peak stress was significantly higher for constructs with embedded fibroblasts. Immunocytochemical and histological analyses demonstrated cell proliferation and ligament-like organization. We have created an engineered tissue that closely approaches key mechanical and viscoelastic properties of the ACL, does not degrade after 20 days in culture, and is histologically similar to the native tissue. This study should aid in developing effective treatments for ACL injury.