Adhesive recognition between developing retinal cells and the optic tecta of the chick embryo.
Adhesive recognition between developing retinal cells and the optic tecta of the chick embryo.
复制标题
发育中的视网膜细胞和鸡胚视神经顶盖之间的粘附识别。
DOI：
10.1016/0012-1606(75)90095-0
复制
发表时间：
1975
影响因子：
2.7
通讯作者：
A. Barbera
中科院分区：
文献类型：
--
作者：
A. Barbera
本文描述了一种可以测量从鸡胚视网膜背侧或腹侧区域到背侧和腹侧顶盖半部分的同位素标记细胞体粘附率的测定方法。视网膜解离后，视网膜腹侧和背侧细胞均优先附着于顶盖腹侧半部分。然而，随着分离后时间的延长，视网膜腹侧细胞的偏好向顶盖背侧半部分转移。无论分离的时间长短，视网膜背侧细胞继续表现出对腹侧顶盖半部分的特异性。当顶盖半部分的发育年龄从8-14天不等时，视网膜细胞对这些顶盖的特异性没有改变。当视网膜年龄不同时，腹侧视网膜细胞直到第6天或更大时才优先附着在背侧顶盖上。背侧视网膜早在第三天就显示出其对腹侧顶盖的特异性。对照实验包括使用非视网膜组织、非神经支配的顶盖半部分和有色视网膜细胞。
An assay is described that can measure the rates of adhesion of isotopically labeled cell bodies from either the dorsal or ventral regions of the chick embryo retina to dorsal and ventral tectal halves. Immediately after dissociation of the retina, both ventral and dorsal retinal cells adhere preferentially to ventral tectal halves. With increasing time after dissociation, however, the preference of the ventral retinal cells shifts to dorsal tectal halves. The dorsal retinal cells continue to show a specificity for ventral tectal halves regardless of the length of time after their dissociation.When the developmental age of the tectal halves is varied from 8–14 days, there is no change in the specificities shown by retinal cells toward these tecta.When retinal age is varied, ventral retinal cells do not adhere preferentially to dorsal tecta until day 6 and older. Dorsal retinas show their specificity toward ventral tecta as early as day three.Control experiments include the use of nonretinal tissues, noninnervated tectal halves and pigmented retinal cells.