Detection of intact influenza viruses using biotinylated biantennary S-sialosides
Detection of intact influenza viruses using biotinylated biantennary S-sialosides
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DOI：
10.1021/ja800842v
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发表时间：
2008-07-02
影响因子：
15
通讯作者：
Lyer, Suri S.
中科院分区：
文献类型：
--
作者：
Kale, Ramesh R.;Mukundan, Harshini;Lyer, Suri S.
我们报告了稳健的生物素化双链唾液酸糖缀合物的模块化合成及其区分两种 A 型流感病毒株的能力。这是在病毒神经氨酸酶的先天酶活性存在的情况下，首次展示了基于糖缀合物的鉴别器捕获和电击完整流感病毒的两个株系。我们还展示了使用糖缀合物作为捕获和报告元件的“carbosassy”，因此不需要抗体。捕获完整的流感病毒对于临床诊断具有潜在的益处。
We report the modular synthesis of robust, biotinaylated biatennary sialylglycoconjugates and their ability to differetiate between two type A influenz strains. This is the first demonstration of glycoconjugate-based discriminator capture and electrion of two strians of intact influenza virus, in the presence of the innate enzymatic activity of viral neuaminidases. We also demonstrate a "carbosassy" using glycoconjugates as capture and reporter elements, which therefore, does not require antibodies. The capture of intact influenz viruses is of potential benetit for clinial diagnostics.