Effect of intravenous substance P on laryngeal adductor activity in young dogs.
Effect of intravenous substance P on laryngeal adductor activity in young dogs.
复制标题
静脉注射 P 物质对幼犬喉内收肌活动的影响。
DOI：
10.1177/000348949910800202
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Luschei,ES
中科院分区：
文献类型：
--
作者：
Bauman,NM;Wang,D;Jaffe,DM;Sandler,AD;Luschei,ES
喉内收反射是喉化学反射和食管喉内收反射的组成部分，这两种反射发生在未成熟的动物中。这两种反射反应也被认为存在于婴儿中，并且可能在导致危及生命的喉痉挛事件以及婴儿猝死综合症中发挥作用。识别介导喉内收的神经递质对于理解反射性喉反应机制和确定潜在的药物预防方法非常重要。 P 物质 (SP) 是一种速激肽，被认为具有感觉神经递质的作用，并可能在介导喉反射中发挥作用。 P物质-免疫反应样纤维和受体存在于喉上皮下组织、迷走神经、结节和颈静脉神经节以及迷走神经脑干核中。在这项研究中，报告了 SP 输注对体内模型中喉部运动活动的影响。 P物质被静脉注射到8只小狗（20至133天龄，平均81.2）中，平均3.0次（范围1至6次）。记录并随后分析心血管、呼吸、动脉血气以及环甲腺（CT）、甲杓肌（TA）和颏舌肌肌电图（EMG）对速激肽输注的反应。在 24 项研究中的 23 项中，SP 输注引起 CT 或 TA EMG 活性显着增加，并且这种增加通常在输注后 60 秒内明显增加。颏舌肌肌电图活动没有增加。伴随着喉部反应，平均动脉压立即显着降低，呼吸频率和胸壁偏移深度增加。动脉血气值保持不变 (p > .05)。当动物用全身性 SP 拮抗剂（辉瑞 CP-96,345）预处理时，喉内收肌对 SP 输注的反应被阻断。这项研究表明，速激肽 SP 的外周输注可导致喉内收肌活动显着增加。该反应可以通过用全身性 SP 拮抗剂预处理动物来阻断。由于 SP 被认为主要充当感觉神经递质，因此这些发现对于理解反射性喉内收肌反应机制可能很重要。
Reflex laryngeal adduction is a component of both the laryngeal chemoreflex and the esophagolaryngeal adductor reflex, two life-threatening reflexes that occur in immature animals. These two reflex responses are also thought to exist in infants and may play a role in causing life-threatening laryngospastic events and perhaps sudden infant death syndrome. Identifying neurotransmitters that mediate laryngeal adduction is important to understanding the mechanism of reflex laryngeal responses and to identifying potential means of pharmacologic prevention. Substance P (SP), a tachykinin, putatively functions as a sensory neurotransmitter and may play a role in mediating laryngeal reflexes. Substance P-immunoreactive-like fibers and receptors are present in the subepithelial tissues of the larynx, the vagus nerves, the nodose and jugular ganglia, and the vagal brain stem nuclei. In this investigation, the effect of SP infusion on laryngeal motor activity in an in vivo model is reported. Substance P was infused intravenously into 8 puppies (20 to 133 days of age, mean 81.2), on a mean of 3.0 occasions (range 1 to 6). Cardiovascular, respiratory, arterial blood gas, and cricothyroid (CT), thyroarytenoid (TA), and genioglossus electromyographic (EMG) responses to infusion of the tachykinin were recorded and subsequently analyzed. The SP infusion induced a marked increase in CT or TA EMG activity in 23 of 24 studies, and the increase was typically apparent within 60 seconds of the infusion. An increase in genioglossus EMG activity did not occur. An immediate, profound decrease in mean arterial pressure and an increase in respiratory rate and depth of chest wall excursion accompanied the laryngeal response. Arterial blood gas values remained unchanged (p > .05). The laryngeal adductor response to SP infusion was blocked when animals were pretreated with a systemic SP antagonist (Pfizer CP-96,345). This study demonstrates that peripheral infusion of the tachykinin SP induces a marked increase in laryngeal adductor activity. The response may be blocked by pretreatment of animals with a systemic SP antagonist. Because SP is thought to act primarily as a sensory neurotransmitter, these findings may be important in understanding the mechanism of reflex laryngeal adductor responses.