Local and chemical distribution of phlorotannins in brown algae
Local and chemical distribution of phlorotannins in brown algae
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DOI：
10.1023/b:japh.0000047781.24993.0a
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发表时间：
2004-08-01
影响因子：
3.3
通讯作者：
Nakamura, T
中科院分区：
文献类型：
--
作者：
Shibata, T;Kawaguchi, S;Nakamura, T
本文研究了海带科海带、双环爱胜子、海带海带和黑刺海带中邻氯单宁的局部分布和化学分布。通过香草醛-盐酸染色的光镜观察，发现无论种类、生长阶段或器官，邻苯二甲酸单宁都积累在菌体外层皮层的营养细胞中。粗邻氯单宁约占每种藻类藻粉的3.0%。高效液相色谱(HPLC)分析表明，该植物的间苯三酚主要由间苯三酚(0.9%)、间苯三酚四聚体(4.4%)、eckol(7.5%)、间苯三酚A(21.9%)、dieckol(23.4%)和8，8‘-bieckol(24.6%)组成，另外还有一些未知的酚类化合物(17.3%)。在海带科植物中，邻苯二甲酸单宁的组成差异不大，但在双叶海带中四聚体的含量明显较高，其相对分子质量为478。
The local and chemical distribution of phlorotannins among the Japanese Laminariaceae, Eisenia bicyclis, Ecklonia cava and Ecklonia kurome, was investigated. As a result of light microscopy observations with vanillin-HC1 staining, phlorotannins were found to be accumulated within the vegetative cells of the outer cortical layer of the thalli, regardless of the species, stage of growth or organ. Crude phlorotannins comprised about 3.0% of the algal powder for each of the algae. High-performance liquid chromatography ( HPLC) showed that the phlorotannins of E. bicyclis were composed of phloroglucinol ( 0.9%), phloroglucinol tetramer ( 4.4%), eckol ( 7.5%), phlorofucofuroeckol A ( 21.9%), dieckol ( 23.4%), and 8,8'-bieckol ( 24.6%), plus some other unknown phenolic compounds ( 17.3%). The composition of the phlorotannins differed little among the Laminariaceae, except for a significantly larger amount of the tetramer, MW 478, in E. bicyclis.