Microbial community PLFA and PHB responses to ecosystem restoration in tallgrass prairie soils
Microbial community PLFA and PHB responses to ecosystem restoration in tallgrass prairie soils
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DOI：
10.1016/j.soilbio.2005.02.033
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发表时间：
2005-10-01
影响因子：
9.7
通讯作者：
White, DC
中科院分区：
文献类型：
--
作者：
McKinley, VL;Peacock, AD;White, DC
北美原住民草原以其土壤肥沃而闻名，具有优良的土壤结构和非常高的有机含量和微生物生物量。在这项研究中，将来自不同年龄和植物群落组成的三个草原恢复区的表层土壤与附近未受干扰的原生草原残余物和种植农田的土壤物理、化学和微生物特性进行了比较。与农田和最近开始的恢复（采样前 7 年）相比，原始草原残余物以及两次长期（21 年和 24 年）草原恢复的土壤湿度、有机质、总碳、总氮、总硫、C:N、持水能力和微生物量（总 PLFA）显着更高（p < 0.05）。农业和最近恢复的地点的土壤容重明显更大（p < 0.05）。在大多数情况下，恢复地点的土壤质量指标和微生物群落结构介于原始草原和农业地点之间。农业和近期恢复地点的聚β羟基丁酸酯（PHB）和PLFA营养应激指标水平显着高于长期恢复地区或原生草原（p < 0.05）。通过对 PLFA 数据的判别分析，可以将样本分配到正确的地点，但两个长期恢复地点重叠的情况除外。冗余分析表明，草原年龄（p < 0.005）是决定PLFA微生物群落组成的最重要的环境因素，C:N（p < 0.015）也很重要。这些发现表明，草原恢复可以改善表层土壤的质量。我们预测，农田转变为草原将使土壤质量、微生物群落生物量和微生物群落组成向原生草原的方向转变，但根据测试的恢复方法，可能需要几十年的时间才能在整个土壤剖面上达到原始草原的水平。 (c) 2005 Elsevier Ltd. 保留所有权利。
Native North American prairie grasslands are renowned for the richness of their soils, having excellent soil structure and very high organic content and microbial biomass. In this study, surface soils from three prairie restorations of varying ages and plant community compositions were compared with a nearby undisturbed native prairie remnant and a cropped agricultural field in terms of soil physical, chemical and microbial properties. Soil moisture, organic matter, total carbon, total nitrogen, total sulfur, C:N, water-holding capacity and microbial biomass (total PLFA) were significantly greater (p < 0.05) in the virgin prairie remnant as well as the two long-term (21 and 24 year) prairie restorations, compared with the agricultural field and the restoration that was begun more recently (7 years prior to sampling). Soil bulk density was significantly greater (p < 0.05) in the agricultural and recently restored sites. In most cases, the soil quality indicators and microbial community structures in the restoration sites were intermediate between those of the virgin prairie and the agricultural sites. Levels of poly-beta-hydroxybutyrate (PHB) and PLFA indicators of nutritional stress were significantly greater (p < 0.05) in the agricultural and recent restoration sites than in the long-term restorations or the native prairie. Samples could be assigned to the correct site by discriminant analysis of the PLFA data, with the exception that the two long-term restoration sites overlapped. Redundancy analysis showed that prairie age (p < 0.005) was the most important environmental factor in determining the PLFA microbial community composition, with C:N (p < 0.015) also being significant. These findings demonstrate that prairie restorations can lead to improved quality of surface soils. We predict that the conversion of farmland into prairie will shift the soil quality, microbial community biomass and microbial community composition in the direction of native prairies, but with the restoration methods tested it may take many decades to approach the levels found in a virgin prairie throughout the soil profile. (c) 2005 Elsevier Ltd. All rights reserved.