Genetic dissection of leaf area by jointing two F2:3 populations in maize (Zea Mays L.)
Genetic dissection of leaf area by jointing two F2:3 populations in maize (Zea Mays L.)
复制标题
DOI：
10.1111/j.1439-0523.2012.01993.x
复制
发表时间：
2012-10-01
期刊：
影响因子：
2
通讯作者：
Chen, Yanhui
中科院分区：
文献类型：
--
作者：
Ku, Lixia;Zhang, Jun;Chen, Yanhui
叶面积是玉米最重要的光合受体，因此，叶面积的大小（特别是穗位三叶）对玉米干物质积累和产量起着至关重要的作用。LA的遗传机制对玉米高产育种具有重要的理论意义。然而，LA的遗传控制在玉米中仍然未知。在这项研究中，LA的QTL定位使用两套连接的F2：3群体与229和245个家庭在两到三个环境中评估。利用Meta分析整合两个群体的遗传图谱并检测QTL。共检测到38个QTL，其中群体1中17个，群体2中21个。38个QTL整合到13个元QTL（mQTL）中。发现新的mQTL 2 -1包括两个初始QTL，mQTL 5 -2包括四个初始QTL。两个QTL都有一个R2大于10%的初始QTL。因此，这两个mQTL的染色体区域具有较高的QTL共定位，可能是LA的重要QTL的热点。结果表明，7个控制LA相关性状的关键候选基因与5个相应的mQTL（Arlng 1、ArPFL 2/OsH 1、ArCLF/ArCyCB 2;3（LL）、ArCYCD 3;2和Osnr 1）一致。特别是，Arlng 1被定位在重要的mQTL 2 -1区间内。该mQTL遗传区域的精细定位值得进一步研究，并可用于标记辅助育种。这些结果为进一步研究和了解LA的分子机制提供了有用的信息。
With 1 figure and 5 tables Abstract Leaf area (LA) is the most important photosynthesis acceptor, and therefore, size of LA (especially the three ear-leaves) plays a vital role in dry matter accumulation and grain yield in maize. Genetic mechanisms on LA have a theoretical significance for breeding maize with high yield. However, the genetic control of LA remains unknown in maize. In this study, QTL for LA were mapped using two sets of connected F2 : 3 populations with 229 and 245 families evaluated in two to three environments. Meta-analysis was used to integrate genetic maps and detect QTL across two populations. A total of 38 QTL were detected, 17 in population 1 and 21 in population 2. Thirty-eight QTL were integrated in 13 meta-QTL (mQTL). The novel mQTL2-1 was found to include two initial QTL, and mQTL5-2 to include four initial QTL. Both of them had one of the initial QTL with R2 over 10%. Therefore, the chromosome regions for these two mQTL with high QTL co-localization might be hot spots for important QTL for LA. Seven key candidate genes controlling related traits of LA were observed to be coincided with five corresponding mQTL, including Arlng1, ArPFL2/OsH1, ArCLF/ArCyCB2;3(LL), ArCYCD3;2, and Osnrl. Especially, Arlng1 was mapped within the important mQTL2-1 interval. Fine mapping for genetic regions of such mQTL is worth further study and could be put into use in marker-assisted breeding. The results provide useful information for further researching and understanding of the molecular mechanisms with regard to LA.