Mitochondrial photodamage and PDT-induced apoptosis
Mitochondrial photodamage and PDT-induced apoptosis
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DOI：
10.1016/s1011-1344(97)00127-9
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发表时间：
1998-02-01
影响因子：
5.4
通讯作者：
Luo, Y
中科院分区：
文献类型：
--
作者：
Kessel, D;Luo, Y
使用四种具有特定靶标（线粒体、溶酶体和质膜）的光敏剂来描述 PDT 诱导小鼠白血病细胞凋亡的机制。使用两种敏化剂进行了其他研究，这两种敏化剂对线粒体和溶酶体均造成光损伤，但膜光损伤方面有所不同。 PDT 在线粒体光损伤后诱导细胞凋亡反应，但在溶酶体或细胞膜选择性损伤后则不诱导细胞凋亡反应。此外，后者可以延迟或抑制线粒体光损伤后细胞凋亡的出现。我们之前曾报道过，细胞暴露于高浓度的卟啉会在黑暗中引起细胞凋亡反应。这也与线粒体损伤有关。这些结果与最近提出的线粒体成分的释放可以引发细胞凋亡反应的建议一致。线粒体光损伤后 ATP 消耗似乎在细胞凋亡程序的启动中没有发挥作用。 (C) 1998 Elsevier Science S.A.
Four photosensitizers with specific targets (mitochondria, lysosomes and plasma membrane) were used to delineate the mechanism of PDT-induced apoptosis in murine leukemia cells. Additional studies were carried out with two sensitizers which caused photodamage to both mitochondria and lysosomes, but varied with regard to membrane photodamage. PDT induced an apoptotic response after mitochondrial photodamage, but not after selective damage to lysosomes or to the cell membrane. Moreover, the latter could delay or inhibit the appearance of apoptosis after mitochondrial photodamage. We had previously reported that exposure of cells to high porphycene concentrations caused an apoptotic response in the dark; this was also associated with mitochondrial damage. These results are consistent with recent proposals that release of mitochondrial components can trigger an apoptotic response. ATP depletion after mitochondrial photodamage does not appear to play a role in initiation of the apoptotic program. (C) 1998 Elsevier Science S.A.