Abnormality of CD4+CD25+ regulatory T cells in idiopathic thrombocytopenic purpura
Abnormality of CD4+CD25+ regulatory T cells in idiopathic thrombocytopenic purpura
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DOI：
10.1111/j.1600-0609.2006.00780.x
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发表时间：
2007-02-01
影响因子：
3.1
通讯作者：
Han, Zhong Chao
中科院分区：
文献类型：
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作者：
Liu, Bin;Zhao, Hui;Han, Zhong Chao
目的：本研究旨在探讨特发性血小板减少性紫癜（ITP）患者外周血中CD4（+）CD25（+）调节性T细胞（Treg细胞）的分布及功能。研究方法：通过流式细胞术分析从健康供体或ITP患者收集的外周血单核细胞中的Treg细胞数。基于DNA合成过程中BrdU掺入的测量，通过酶联免疫吸附测定试剂盒测定细胞增殖的定量。结果如下：与缓解期患者（11.63 ± 4.56%）和健康受试者（12.68 ± 3.59%）相比，活动期和非缓解期ITP患者的Treg细胞百分比（5.79 ± 1.22%）显著降低。还发现ITP患者中Treg细胞的抑制活性受损。结论：提示Treg细胞数量和功能的下降可能是ITP免疫调节功能紊乱的机制之一。
Objectives: The aim of this study was to explore the profile and function of CD4(+)CD25(+) regulatory T cells (Treg cells) in idiopathic thrombocytopenic purpura (ITP) patients. Methods: Treg cell numbers were analyzed by flow cytometric analysis in peripheral blood mononuclear cells collected from healthy donors or patients with ITP. Quantification of cell proliferation was assayed by an enzyme-linked immunosorbent assay kit, based on the measurement of BrdU incorporation during DNA synthesis. Results: The percentage of Treg cells was significantly decreased in ITP patients in active and non-remission state(5.79 +/- 1.22%) when compared with the patients in remission(11.63 +/- 4.56%) and to healthy subjects(12.68 +/- 3.59%). The suppressive activity of Treg cells in ITP patients was also found to be impaired. Conclusion: These results suggest that decreased number and function of Treg cells might be one of mechanisms that cause immune regulation dysfunction in ITP.