Novel technologies for the discovery and quantitation of biomarkers of toxicity
Novel technologies for the discovery and quantitation of biomarkers of toxicity
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DOI：
10.1016/j.tox.2007.11.020
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发表时间：
2008-03-20
期刊：
影响因子：
4.5
通讯作者：
Vaidya, Vishal S.
中科院分区：
文献类型：
--
作者：
Collings, Fitz B.;Vaidya, Vishal S.
可靠的毒性生物标志物对于安全进行临床前和临床试验都是必要的，并且对于准确临床评估可能导致组织损伤的治疗方案也越来越需要。技术的最新进展为生物标志物筛选工具包增加了几种新工具，并提高了现有定量分析的通量。基因组学、蛋白质组学和代谢组学为寻找预测性、特异性生物标志物提供了丰富的数据。基于多重ELISA的分析系统、硅纳米线阵列和图案化纸提供了在宽动态范围内快速、有效的样品分析的独特能力。强大的集成系统生物学软件和不断增长的开源数据存储库提供了共享，减少和分析多个来源数据的新方法。本文综述的新技术有可能显着减少检测时间和成本，并提高候选毒性生物标志物筛选方法的灵敏度。(C)2007爱思唯尔爱尔兰有限公司保留所有权利。
Reliable biomarkers of toxicity are necessary both for the safe conduct of pre-clinical and clinical trials, and are increasingly needed for accurate clinical evaluation of treatment regimens with the potential to cause tissue injury. Recent advances in technology have added several new tools to the biomarker screening toolkit and improved the throughput of existing quantitative assays. Genomics, proteomics, and metabolomics have provided a wealth of data in the search for predictive, specific biomarkers. Multiplexed ELISA-based assay systems, silicon nanowire arrays, and patterned paper present unique abilities for fast, efficient sample analysis over a broad dynamic range. Powerful integrative systems biology software and growing open-source data repositories offer new ways to share, reduce, and analyze data from multiple sources. Novel technologies reviewed here have the potential to significantly reduce assay time and cost and improve the sensitivity of screening methods for candidate biomarkers of toxicity. (C) 2007 Elsevier Ireland Ltd. All rights reserved.