Chemotactic movement and attachment of Agrobacterium tumefaciens to banana cells and tissues
Chemotactic movement and attachment of Agrobacterium tumefaciens to banana cells and tissues
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DOI：
10.1016/s0176-1617(99)80014-5
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发表时间：
1999-08-01
影响因子：
4.3
通讯作者：
Sági, L
中科院分区：
文献类型：
--
作者：
Hernández, JBP;Remy, S;Sági, L
为了阐明农杆菌介导的香蕉高效转化，研究了植物-细菌相互作用的初始步骤。用琼脂平板法研究了土壤杆菌对组织的趋化性。用不同香蕉地方品种的离体叶、根茎、根和离体增殖组织，均能诱导A.可以通过广泛的创伤来增强。通过紫外荧光显微镜和扫描电子显微镜检查农杆菌附着，这表明细菌单独结合或大量附着到单个香蕉细胞和组织的表面的存在。可以得出结论，至少在相互作用A的两个早期步骤期间。根癌农杆菌似乎与香蕉相容，表明具有遗传转化的潜力。
For the elaboration of efficient Agrobacterium-mediated transformation of banana the initial steps in the plant-bacterium interaction were investigated. Chemotaxis of agrobacteria towards tissues was studied using a swarm agar plate system. Excised leaf, rhizome, root and in vitro proliferating tissues from different banana landraces were found to be able to elicit a positive chemotactic reaction of A. tumefaciens that could be enhanced by extensive wounding. Agrobacterium attachment was examined by UV fluorescence microscopy and scanning electron microscopy, which demonstrated the presence of bacteria individually bound or massively attached to the surface of single banana cells and tissues. It can be concluded that at least during the two early steps of interaction A. tumefaciens appears to be compatible with banana, indicating the potential for genetic transformation.