The interplay of DNA binding, ATP hydrolysis and helicase activities of the archaeal MCM helicase
The interplay of DNA binding, ATP hydrolysis and helicase activities of the archaeal MCM helicase
复制标题
DOI：
10.1042/bj20110084
复制
发表时间：
2011-06-01
影响因子：
4.1
通讯作者：
Bell, Stephen D.
中科院分区：
文献类型：
--
作者：
Liew, Li Phing;Bell, Stephen D.
古细菌的 MCM（微型染色体维持）蛋白被广泛认为是这些生物体的复制 DNA 解旋酶。大多数古细菌都拥有单一的 MCM 直系同源物，它与被认为是活性形式的单一六聚体形成同源多聚体组装体。在本研究中，我们表征了超嗜热古菌硫磺硫化叶菌 (Sulfolobus solfataricus) 的 MCM ATP 酶结构域中高度保守残基的作用。我们的结果确定了将 DNA 结合信息传递到 ATP 酶活性位点的潜在渠道。
The MCM (minichromosome maintenance) proteins of archaea are widely believed to be the replicative DNA helicase of these organisms. Most archaea possess a single MCM orthologue that forms homo-multimeric assemblies with a single hexamer believed to be the active form. In the present study we characterize the roles of highly conserved residues in the ATPase domain of the MCM of the hyperthermophilic archaeon Sulfolobus solfataricus. Our results identify a potential conduit for communicating DNA-binding information to the ATPase active site.