Rat kinesin light chain 3 associates with spermatid mitochondria.
Rat kinesin light chain 3 associates with spermatid mitochondria.
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DOI：
10.1016/j.ydbio.2004.07.014
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发表时间：
2004-11
影响因子：
2.7
通讯作者：
Ying Zhang;R. Oko;F. A. van der Hoorn
中科院分区：
文献类型：
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作者：
Ying Zhang;R. Oko;F. A. van der Hoorn
我们最近发现，在大鼠精子细胞，驱动蛋白轻链KLC 3可以与外部致密纤维，主要精子尾部组件，并积累在精子中段。在这里，我们表明，从大鼠精子细胞中分离的线粒体已绑定KLC 3。免疫电子显微镜表明，该协会的KLC 3与线粒体相一致的阶段，在精子发生时，线粒体从质膜到发展中的中段。KLC 3能够在体外结合来自精子细胞以及体细胞的线粒体，其采用保守的驱动蛋白轻链基序，即四肽重复序列。KLC 3在成纤维细胞中的表达导致靠近细胞核的大KLC 3簇的形成，其也包含线粒体：在这些簇中不存在其他细胞器。线粒体不存在于KLC 3集群后删除KLC 3的tetratrico-肽重复。我们的结果表明，大鼠精子细胞驱动蛋白轻链KLC 3可以与线粒体结合。
We recently discovered that in rat spermatids, kinesin light chain KLC3 can associate with outer dense fibers, major sperm tail components, and accumulates in the sperm midpiece. Here, we show that mitochondria isolated from rat-elongating spermatids have bound KLC3. Immunoelectron microscopy indicates that the association of KLC3 with mitochondria coincides with the stage in spermatogenesis when mitochondria move from the plasma membrane to the developing midpiece. KLC3 is able to bind in vitro to mitochondria from spermatids as well as somatic cells employing a conserved kinesin light chain motif, the tetratrico-peptide repeats. Expression of KLC3 in fibroblasts results in formation of large KLC3 clusters close to the nucleus, which also contain mitochondria: no other organelles were present in these clusters. Mitochondria are not present in KLC3 clusters after deletion of KLC3's tetratrico-peptide repeats. Our results indicate that the rat spermatid kinesin light chain KLC3 can associate with mitochondria.