Eye orientation during static tilts and its relationship to spontaneous head pitch in the laboratory mouse
Eye orientation during static tilts and its relationship to spontaneous head pitch in the laboratory mouse
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DOI：
10.1016/j.brainres.2007.11.053
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发表时间：
2008-02-08
期刊：
影响因子：
2.9
通讯作者：
Stahl, John S.
中科院分区：
文献类型：
--
作者：
Oommen, Brian S.;Stahl, John S.
眼位和头部定向均受黄斑（耳石）器官的影响，分别通过倾斜黄斑眼反射（tiltMOR）和前庭-颈反射。控制头部位置的机构也影响眼睛的静止位置，因为头部定向通过倾斜MOR影响眼睛位置。尽管越来越受欢迎的小鼠的前庭和眼运动功能的研究，相对知之甚少，在这个物种的倾斜MOR，自发方向的头部，以及它们之间的相互关系。我们使用2D视频眼电图来确定在C57 BL/6小鼠的绝对水平和垂直位置的眼睛在身体方向跨越360度的俯仰和滚动轴。我们还在相同的动物中测定了Ambestion期间的头俯仰。眼仰角随俯仰角或滚转角的变化近似呈正弦曲线。在中央+/-30度的俯仰上，光中的灵敏度和增益分别为31.7度/g和0.53。滚动的相应值分别为31.5度/g和0.52度/g。在光明和黑暗中采用的绝对位置仅略有不同。在Ambatum期间，小鼠以29度的向下倾斜携带前额叶-前囟平面，对应于64度的水平眼位置和22度的垂直眼位置。垂直位置靠近由俯仰倾斜MOR产生的眼睛运动范围的中心。结果表明，倾斜MOR在该物种中是稳健的，并且有利于跨实验室标准化俯仰方向。强大的tiltMOR也有显着的方法学意义的实践中的瞳孔跟踪视频眼电在这个物种。由爱思唯尔公司出版
Both eye position and head orientation are influenced by the macular (otolith) organs, via the tilt maculo-ocular reflex (tiltMOR) and the vestibulo-collic reflexes, respectively. The mechanisms that control head position also influence the rest position of the eye because head orientation influences eye position through the tiltMOR. Despite the increasing popularity of mice for studies of vestibular and ocular motor functions, relatively little is known in this species about tiltMOR, spontaneous orientation of the head, and their interrelationship. We used 2D video oculography to determine in C57BL/6 mice the absolute horizontal and vertical positions of the eyes over body orientations spanning 360 degrees about the pitch and roll axes. We also determined head pitch during ambulation in the same animals. Eye elevation varied approximately sinusoidally as functions of pitch or roll angle. Over the central +/- 30 degrees of pitch, sensitivity and gain in the light were 31.7 degrees/g and 0.53, respectively. The corresponding values for roll were 31.5 degrees/g and 0.52. Absolute positions adopted in light and darkness differed only slightly. During ambulation, mice carried the lambda-bregma plane at a downward pitch of 29 degrees, corresponding to a horizontal eye position of 64 degrees and a vertical eye position of 22 degrees. The vertical position is near the center of the range of eye movements produced by the pitch tiltMOR. The results indicate that the tiltMOR is robust in this species and favor standardizing pitch orientation across laboratories. The robust tiltMOR also has significant methodological implications for the practice of pupil-tracking video oculography in this species. Published by Elsevier B.V.