Novel cuticular proteins revealed by the honey bee genome
Novel cuticular proteins revealed by the honey bee genome
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DOI：
10.1016/j.ibmb.2006.10.009
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发表时间：
2007-02-01
影响因子：
3.8
通讯作者：
Maleszka, R.
中科院分区：
文献类型：
--
作者：
Kucharski, R.;Maleszka, J.;Maleszka, R.
随着蜜蜂基因组计划的完成，现在正从获取基因序列过渡到了解基因组中基因产物的作用和背景。在这里，我们注释和特点的三个基因在一个GC丰富的11 kb的基因组区域上的连接组4编码高度疏水性的多肽（命名为apidermins; APD 1-3）包含两个序列基序特征的表皮蛋白和明显的新功能。五种氨基酸Ala、Gly、Leu、Pro和瓦尔占其各自序列的74-86%，其中Ala是最丰富的残基（每种肽的至少30%）。一个保守的四肽AAPA/V被发现在所有三种蛋白质，但没有一个有'R和R'的签名涉及几丁质结合。APD-1和APD-2两种蛋白质在成熟蛋白质的N-末端附近的短的非疏水性延伸中含有富含精氨酸的基序RERR，并且在这两种蛋白质中色氨酸是C-末端残基。所有这三个基因都是剪接的，并以确定的时空模式高度表达。APD-1在外骨骼表皮中表达，但仅在蛹晚期和成虫早期的几天的有限时期内表达，此时角质层变暗。APD 2似乎是“内部”角质层的一种蛋白质，在气管、食道和胃以及胚胎中表达。apd-3的表达与apd-1和apd-3的表达部分重叠，但apd-3也与非色素性角质层如白色脓疱的眼盖和外角质层独特相关。这项研究扩大了收集的基因编码表皮蛋白的三个新的和良好的特点成员。(c)2006爱思唯尔有限公司保留所有权利。
With the completion of the honey bee genome project, a transition is now occurring from the acquisition of gene sequence to understanding the role and context of gene products within the genome. Here we annotated and characterised a cluster of three genes in a GC-rich 11 kb genomic region on the linkage group 4 encoding highly hydrophobic polypeptides (named apidermins; APD 1-3) containing both sequence motifs characteristic of cuticular proteins and distinctly novel features. Five amino acids, Ala, Gly, Leu, Pro and Val, account for 74-86% of their respective sequences with Ala being the most abundant residue (at least 30% of each peptide). A conserved tetra-peptide AAPA/V is found in all three proteins, but none has the 'R and R' signature implicated in chitin binding. Two proteins, APD-1 and APD-2, contain an arginine-rich motif RERR in short non-hydrophobic stretches near the N-terminal of mature proteins and in both proteins tryptophan is the C-terminal residue. All three genes are spliced and highly expressed in a defined spatio-temporal pattern. apd-1 is expressed in the exoskeletal epidermis, but only during a restricted period of a few days of late pupal and early adult life when the cuticle becomes dark. APD2 appears to be a protein of "internal" cuticles and is expressed in the tracheas, oesophagus and stomach, and also in the embryo. The expression of apd-3 partly overlaps with both apd-1 and apd-3, but apd-3 also is uniquely associated with non-pigmented cuticles such as the eye cover and external cuticle of white pupae. This study expands the collection of genes encoding cuticular proteins by three novel and well characterised members. (c) 2006 Elsevier Ltd. All rights reserved.