Rearing tardigrades: Results and problems
Rearing tardigrades: Results and problems
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DOI：
10.1078/0044-5231-00028
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发表时间：
2001-12-01
影响因子：
1.4
通讯作者：
Rebecchi, L
中科院分区：
文献类型：
--
作者：
Altiero, T;Rebecchi, L
我们报告了我们的第一个结果，试图饲养四种eutardigrates居住在不同的基板，喂养不同种类的食物，其特点是不同的性条件和繁殖模式。尝试进行了以下个别陆生食肉动物（Macrobiotus richtersi，M。（Diphascon cf. scoticum; Isohypsibius monoicus）种。食肉叶凋落物居住的物种饲养在含有琼脂作为底物和食菌线虫作为食物的小盘子。用三倍体获得了5个世代。产雌M. richtersi，而雌雄同体的M. Joanziran，Diphascon cf.苏格兰和我在含有藻类作为食物和底物的小碟中饲养monocicus。这两个物种都获得了几个世代。在D中从未发现雄性。参见苏格兰和我monoicus是雌雄同体的。从孵化分离的标本保持和繁殖的两个物种，证明孤雌生殖在第一个和自受精在后者。讨论了缓步动物饲养中存在的问题和未来的应用前景。
We report our first results of attempts to rear four species of eutardigrades inhabiting different substrates, feeding on different kinds of food and characterized by different sexual conditions and modes of reproduction. Attempts were carried out to follow individual terrestrial carnivorous (Macrobiotus richtersi, M. joannae) and limnic herbivorous (Diphascon cf. scoticum; Isohypsibius monoicus) species. Carnivorous leaf litter-dwelling species were reared in small dishes containing agar as substrate and bacteriophagous nematodes as food. Five generations were obtained with the triploid. thelytokous strain of M. richtersi, whereas three generations were obtained with the hermaphrodite species M. joannae, Diphascon cf. scoticum and I. monoicus were reared in small dishes containing algae as food and substrate. Several generations were obtained for both species. Males were never found in D. cf. scoticum and I. monoicus was hermaphroditic. Specimens isolated from hatchings were maintained and reproduced in both species, demonstrating parthenogenesis in the first one and self-fertilization in the latter. Consideration of the problems and on the future applications of tardigrade rearing are discussed.