MdbHLH3 modulates apple soluble sugar content by activating phosphofructokinase gene expression
MdbHLH3 modulates apple soluble sugar content by activating phosphofructokinase gene expression
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MdbHLH3通过激活磷酸果糖激酶基因表达调节苹果可溶性糖含量
DOI：
10.1111/jipb.13236
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发表时间：
2022
影响因子：
11.4
通讯作者：
Hu Dagang
中科院分区：
文献类型：
--
作者：
Yu Jianqiang;Gu Kaidi;Zhang Lili;Sun Cuihui;Zhang Quanyan;Wang Jiahui;Wang Chukun;Wang Wenyan;Du Mengchi;Hu Dagang
本文报道了焦磷酸依赖性磷酸果糖激酶基因MdPFPβ通过提高苹果光合性能和糖代谢酶活性来调控可溶性糖积累的研究结果。生化分析表明，一个碱性螺旋-环-螺旋（bHLH）转录因子MdbHLH 3与MdPFPβ启动子结合并激活其表达，从而促进苹果果实中可溶性糖的积累。此外，MdPFPβ在番茄中的过表达影响了植物的光合作用和碳代谢。我们确定MdbHLH 3通过激活MdPFPβ表达来增加苹果的光合速率和可溶性糖积累。我们的结果为我们提供了线索MdbHLH 3通过激活MdPFPβ基因的表达和协调碳水化合物的分配来调节可溶性糖的积累。
Sugars are involved in plant growth,fruit quality,and signaling perception.Therefore,understanding the mechanisms involved in soluble sugar accumulation is essential to understand fruit development.Here,we report that MdPFPβ,a pyrophosphatedependent phosphofructokinase gene,regulates soluble sugar accumulation by enhancing the photosynthetic performance and sugar-metabolizing enzyme activities in apple (Malus domestica Borkh.).Biochemical analysis revealed that a basic helix-loop-helix (bHLH) transcription factor,MdbHLH3,binds to the MdPFPβ promoter and activates its expression,thus promoting soluble sugar accumulation in apple fruit.In addition,MdPFPβ overexpression in tomato influenced photosynthesis and carbon metabolism in the plant.Furthermore,we determined that MdbHLH3 increases photosynthetic rates and soluble sugar accumulation in apple by activating MdPFPβ expression.Our results thus shed light on the mechanism of soluble sugar accumulation in apple leaves and fruit:MdbHLH3 regulates soluble sugar accumulation by activating MdPFPβ gene expression and coordinating carbohydrate allocation.