Identification of plant stress-responsive determinants in arabidopsis by large-scale forward genetic screens
Identification of plant stress-responsive determinants in arabidopsis by large-scale forward genetic screens
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DOI：
10.1093/jxb/erj093
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发表时间：
2006-03-01
影响因子：
6.9
通讯作者：
Hasegawa, PM
中科院分区：
文献类型：
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作者：
Koiwa, H;Bressan, RA;Hasegawa, PM
所有植物都能感知并适应不利的环境条件，然而，与野生近缘种相比，农作物在非生物胁迫耐受性方面表现出较少的遗传多样性，这表明存在胁迫适应性的遗传基础。模型植物遗传系统和目前可用的大量分子遗传资源极大地增强了我们识别非生物胁迫响应遗传决定因素的能力。在生态型 C24(RD29a-LUC) 和 Col-0 gl1 sos3-1 遗传背景下对 T-DNA 诱变的拟南芥种群进行正向遗传筛选，以开始对此类决定因素进行详尽的搜索。 C24(RD29a-LUC)筛选鉴定出盐/渗透胁迫敏感性改变的突变体或盐/渗透/冷/ABA响应性RD29a基因表达改变的突变体。此外，还筛选了改变 sos3-1 的 NaCl 敏感性的突变，以寻找 SOS3 介导的盐胁迫反应的潜在遗传抑制子或增强子。总共筛选了超过 250,000 个独立插入系，并恢复了超过 200 个表现出改变的应激/ABA 反应的个体突变体。尽管其中一些突变体已被报道，但大多数尚未得到详细研究。值得注意的例子包括 SOS1 的新等位基因以及编码寡糖转移酶 STT3a 亚基、突触融合蛋白、RNA 聚合酶 II CTD 磷酸酶、转录因子、ABA 生物合成酶、Na+ 转运蛋白 HKT1 和 SUMO E3 连接酶的基因突变。参与许多基本细胞功能的基因突变的应激特异性表型表明了参与应激适应的细胞稳态的广泛控制机制。
All plants sense and adapt to adverse environmental conditions, however, crop plants exhibit less genetic diversity for abiotic stress tolerance than do wild relatives indicating that a genetic basis exists for stress adaptability. Model plant genetic systems and the plethora of molecular genetic resources that are currently available are greatly enhancing our ability to identify abiotic stress-responsive genetic determinants. Forward genetic screens of T-DNA mutagenized Arabidopsis thaliana populations in the genetic background of ecotypes C24(RD29a-LUC) and Col-0 gl1 sos3-1 were carried out to begin an exhaustive search for such determinants. The C24(RD29a-LUC) screens identified mutants with altered salt/osmotic stress sensitivity or mutants with altered expression of the salt/osmotic/cold/ABA-responsive RD29a gene. Also, mutations that alter the NaCl sensitivity of sos3-1 were screened for potential genetic suppressors or enhancers of salt-stress responses mediated by SOS3. In total, more than 250 000 independent insertion lines were screened and greater than 200 individual mutants that exhibited altered stress/ABA responses were recovered. Although several of these mutants have been reported, most have not yet been studied in detail. Notable examples include novel alleles of SOS1 and mutations to genes encoding the STT3a subunit of the oligosaccharyltransferase, syntaxin, RNA polymerase II CTD phosphatases, transcription factors, ABA biosynthetic enzyme, Na+ transporter HKT1, and SUMO E3 ligase. The stress-specific phenotypes of mutations to genes that are involved in many basic cellular functions provide indication of the wide range of control mechanisms in cellular homeostasis that are involved in stress adaptation.