cDNA cloning and in situ hybridization of a novel lysozyme in the Pacific oyster, Crassostrea gigas
cDNA cloning and in situ hybridization of a novel lysozyme in the Pacific oyster, Crassostrea gigas
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DOI：
10.1016/j.cbpb.2007.05.005
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发表时间：
2007-10-01
影响因子：
2.2
通讯作者：
Takahashi, Keisuke G.
中科院分区：
文献类型：
--
作者：
Itoh, Naoki;Takahashi, Keisuke G.
从太平洋牡蛎长牡蛎（Crassostrea gigas）中鉴定出一个新的溶菌酶cDNA。从太平洋牡蛎中提取的第二种溶菌酶被命名为CGL-2。CGL-2 cDNA全长536bp，其中429个开放阅读框编码147个氨基酸残基。估计的CGL-2分子特征（等电点和肽识别位点数量）与东部牡蛎（Crassostrea virginica）的消化溶菌酶cv-溶菌酶2相似。此外，CGL-2在系统发育上与cv-溶菌酶2同源，表明CGL-2和cv-溶菌酶2从同一祖先蛋白进化而来，以适应消化环境。原位杂交结果显示，CGL-2基因在消化细胞中表达。值得注意的是，另一种太平洋牡蛎溶菌酶CGL-1也在相同的细胞中转录。消化憩室中多种溶菌酶的存在和表达表明，CGL-1和CGL-2可能在消化器官中起互补作用。(C) 2007爱思唯尔公司版权所有。
A novel lysozyme cDNA from the Pacific oyster, Crassostrea gigas, was identified. This second lysozyme from the Pacific oyster was designated as CGL-2. The complete CGL-2 cDNA sequence comprises of 536 bp, and 429 by of the open reading frame encodes 147 by of amino acid residues. Estimated CGL-2 molecular characteristics (isoelectric point and numbers of peptide recognition sites) resembled those of cv-lysozyme 2, a digestive lysozyme of the eastern oyster, Crassostrea virginica. Moreover, CGL-2 is phylogenetically homologous to the cv-lysozyme 2, indicating that CGL-2 and cv-lysozyme 2 evolved from the same ancestor protein for adaptation to the digestive environment. In situ hybridization revealed that the CGL-2 gene is expressed in digestive cells. It is noteworthy that the other Pacific oyster lysozyme, CGL-1, was also transcribed in the same cells. Presence and expression of multiple lysozymes in the digestive diverticula suggest that CGL-1 and CGL-2 might play complementary roles in digestive organs. (C) 2007 Elsevier Inc. All rights reserved.