Covalent attachment of a three-dimensionally printed thermoplast to a gelatin hydrogel for mechanically enhanced cartilage constructs
Covalent attachment of a three-dimensionally printed thermoplast to a gelatin hydrogel for mechanically enhanced cartilage constructs
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DOI：
10.1016/j.actbio.2014.02.041
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发表时间：
2014-06-01
影响因子：
9.7
通讯作者：
Malda, Jos
中科院分区：
文献类型：
--
作者：
Boere, Kristel W. M.;Visser, Jetze;Malda, Jos
水凝胶可以为包埋细胞的组织形成提供合适的环境，这使得它们适用于再生医学。然而，水凝胶仅具有有限的机械强度，因此必须增强以用于承载条件下的应用。在大多数方法中，增强组分和水凝胶网络具有差的相互作用，并且两种材料对机械性能的协同效应是无效的。因此，在本研究中，聚（羟甲基乙交酯-co-ε-己内酯）/聚（ε-己内酯）（pHMGCL/PCL）的热塑性聚合物共混物官能化与甲基丙烯酸酯基团（pMHMGCL/PCL）和共价接枝到明胶甲基丙烯酰胺（gelMA）水凝胶通过光聚合。接枝导致水凝胶和热塑性聚合物材料之间的界面结合强度增加至少五倍。用三维打印的pHMGCL/PCL和pMHMGCL/PCL支架增强GelMA结构，并在局灶性关节软骨缺损模型中进行测试。在该模型中，两种材料界面处的共价键导致结构对重复轴向力和旋转力的抵抗力提高。此外，软骨细胞嵌入的结构能够形成软骨特异性基质在体外和体内。因此，通过移植不同材料的界面，可以工程化更强的混合软骨构造。(C)2014 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Hydrogels can provide a suitable environment for tissue formation by embedded cells, which makes them suitable for applications in regenerative medicine. However, hydrogels possess only limited mechanical strength, and must therefore be reinforced for applications in load-bearing conditions. In most approaches the reinforcing component and the hydrogel network have poor interactions and the synergetic effect of both materials on the mechanical properties is not effective. Therefore, in the present study, a thermoplastic polymer blend of poly(hydroxymethylglycolide-co-epsilon-caprolactone)/poly(epsilon-caprolactone) (pHMGCL/PCL) was functionalized with methacrylate groups (pMHMGCL/PCL) and covalently grafted to gelatin methacrylamide (gelMA) hydrogel through photopolymerization. The grafting resulted in an at least fivefold increase in interface-binding strength between the hydrogel and the thermoplastic polymer material. GelMA constructs were reinforced with three-dimensionally printed pHMGCL/PCL and pMHMGCL/PCL scaffolds and tested in a model for a focal articular cartilage defect. In this model, covalent bonds at the interface of the two materials resulted in constructs with an improved resistance to repeated axial and rotational forces. Moreover, chondrocytes embedded within the constructs were able to form cartilage-specific matrix both in vitro and in vivo. Thus, by grafting the interface of different materials, stronger hybrid cartilage constructs can be engineered. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.