CpG motif-based adjuvant as a replacement for Freund's complete adjuvant in a recombinant LHRH vaccine
CpG motif-based adjuvant as a replacement for Freund's complete adjuvant in a recombinant LHRH vaccine
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DOI：
10.1016/j.vaccine.2007.12.010
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发表时间：
2008-02-13
期刊：
影响因子：
5.5
通讯作者：
Reeves, Jerry J.
中科院分区：
文献类型：
--
作者：
Conforti, Valeria A.;De Avila, David M.;Reeves, Jerry J.
本研究比较了：(1)Freund's完全佐剂和CpG oligodeoxynucleotide (ODN) 2006在油包水乳剂中作为佐剂；(2)增加重组卵黄蛋白- lhrh （ova-LHRH）融合蛋白作为避孕疫苗抗原的剂量。各处理组（n = 8头/组）分别为：1只未经处理的对照组；5组接受不同剂量ova-LHRH（1.5、2.3、3.4、5.1、7.6 mg）的CpG ODN；另一组接受3.4毫克的ova-LHRH。母牛在第0周和第14周接种疫苗。所有疫苗治疗均引起性腺功能减退和发情抑制。CpG ODN是LHRH免疫的合适替代品。(c) 2007 Elsevier Ltd.版权所有。
This study compared: (1) Freund's complete adjuvant and CpG oligodeoxynucleotide (ODN) 2006 in water-in-oil emulsion as adjuvants; and (2) increasing doses of a recombinant ovalbumin-LHRH (ova-LHRH) fusion protein as an antigen for a contraceptive vaccine. Treatment groups (n = 8 heifers/group) were: one untreated control group; five groups receiving CpG ODN with different doses of ova-LHRH (1.5; 2.3; 3.4; 5.1; and 7.6 mg); and one group receiving 3.4 mg ova-LHRH in Freund's. Heifers were immunized at weeks 0 and 14. Ail vaccine treatments caused gonadal regression and estrus suppression. CpG ODN is a suitable replacement for Freund's for LHRH immunization. (c) 2007 Elsevier Ltd. All rights reserved.