Ultrasound-mediated gene delivery: Influence of contrast agent on transfection
Ultrasound-mediated gene delivery: Influence of contrast agent on transfection
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DOI：
10.1021/bc0602432
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发表时间：
2007-05-01
影响因子：
4.7
通讯作者：
Bettinger, Thierry
中科院分区：
文献类型：
--
作者：
Mehier-Humbert, Sophie;Yan, Feng;Bettinger, Thierry
在存在不同超声造影剂 (UCA) 和编码绿色荧光蛋白的质粒的情况下，以 1.15 和 2.25 MHz 的超声频率对 MAT B III 细胞进行声处理。通过流式细胞术评估转染效率，同时使用光学和扫描电子显微镜评估超声造影剂破坏。研究发现UCA的气体和壳特性对细胞转染有重要影响。观察到气泡破坏和转染率之间存在良好的相关性，并且首次证明硬壳造影剂（气体微胶囊）是超声介导的基因递送的有希望的候选者。
MAT B III cells were insonified at ultrasound frequencies of 1.15 and 2.25 MHz in the presence of different ultrasound contrast agents (UCA) and a plasmid encoding for the green fluorescent protein. The transfection efficiency was assessed by flow cytometry, while contrast agent destruction by ultrasound was evaluated using optical and scanning electronic microscopies. It was found that the gas and shell properties of the UCA have an important influence on cell transfection. A good correlation was observed between bubble destruction and transfection rate, and it was demonstrated, for the first time, that hard-shelled contrast agents (gas microcapsules) are promising candidates for ultrasound-mediated gene delivery.