Oral and sub-cutaneous vaccination of commercial pigs with a recombinant porcine adenovirus expressing the classical swine fever virus gp55 gene
Oral and sub-cutaneous vaccination of commercial pigs with a recombinant porcine adenovirus expressing the classical swine fever virus gp55 gene
复制标题
DOI：
10.1007/s007050170064
复制
发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Johnson, MA
中科院分区：
文献类型：
--
作者：
Hammond, JM;Jansen, ES;Johnson, MA
将表达猪瘟病毒（CSFV）gp 55/E2基因的重组猪腺病毒通过口服或皮下途径给予市售猪，并测定其对CSFV口服和皮下攻击的敏感性。100%接种疫苗和皮下攻毒的动物受到保护。在经口或皮下接种然后经口攻毒的猪组中，60%的动物受到保护。攻毒前，在60%皮下接种疫苗的猪中检测到CSFV中和抗体，但在口服疫苗的猪中没有检测到CSFV中和抗体。CSFV抗原存在于口服疫苗的存活猪的脾脏中。相比之下，皮下接种显示排除了攻毒存活动物脾脏中CSFV的存在。
A recombinant porcine adenovirus expressing the classical swine fever virus (CSFV) gp55/E2 gene was administered to commercially available pigs via oral or subcutaneous routes and their susceptibility to oral and subcutaneous challenge with CSFV was determined. 100% of animals vaccinated and challenged subcutaneously were protected. In the groups of pigs vaccinated either orally or subcutaneously and then challenged orally, 60% of animals were protected. Before challenge, neutralising antibodies to CSFV were detected in 60% of pigs vaccinated subcutaneously, but in none of those given the vaccine orally. CSFV antigen was found in the spleens of surviving pigs that had been vaccinated orally. In contrast, subcutaneous vaccination was shown to preclude the presence of CSFV in the spleen of animals that survived challenge.