Preparation, Purification, and Use of Fatty Acid-containing Liposomes
Preparation, Purification, and Use of Fatty Acid-containing Liposomes
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DOI：
10.3791/57324
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发表时间：
2018-02-01
影响因子：
1.2
通讯作者：
Szostak, Jack W.
中科院分区：
文献类型：
--
作者：
Jin, Lin;Engelhart, Aaron E.;Szostak, Jack W.
含有单链两亲物，特别是脂肪酸的脂质体，由于这些两亲物的独特化学性质，与含有二酰基磷脂的脂质体相比表现出不同的性质。特别是，由于单链两亲物的相对高的溶解度，脂肪酸脂质体增强动态特性。类似地，含有游离脂肪酸的脂质体对盐和二价阳离子更敏感，这是由于羧酸头部基团与金属离子之间的强相互作用。在此，我们说明了用于制备、纯化和使用包含部分或全部单链两亲物（例如，油酸）。
Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of these amphiphiles. In particular, fatty acid liposomes enhance dynamic character, due to the relatively high solubility of single-chain amphiphiles. Similarly, liposomes containing free fatty acids are more sensitive to salt and divalent cations, due to the strong interactions between the carboxylic acid head groups and metal ions. Here we illustrate techniques for preparation, purification, and use of liposomes comprised in part or whole of single chain amphiphiles (e.g., oleic acids).