Automatic generation system of cell-sized liposomes
Automatic generation system of cell-sized liposomes
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DOI：
10.1016/j.snb.2019.04.096
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发表时间：
2019-08-01
影响因子：
8.4
通讯作者：
Takeuchi, Shoji
中科院分区：
文献类型：
--
作者：
Gotanda, Masahide;Kamiya, Koki;Takeuchi, Shoji
利用微流控技术制备了脂质体和脂质囊泡等人造细胞膜。这些技术在生成细胞大小的脂质体方面具有优势，包括单分散和不对称的脂质组成。细胞大小的不对称脂质体具有各种不对称脂小叶的组合，通过手动操作，使用改进的脉冲喷射装置将六个孔布置在圆形旋转台上，依次产生。在这项研究中，我们揭示了使用步进电机驱动的自动顺序脂质体生成系统形成细胞大小脂质体的最佳转速和喷射时间参数。首先，在微机控制下，优化步进电机的转速，实现平面脂质双分子层的形成。接下来，我们使用该自动化系统以优化的旋转速度生成细胞大小的脂质体。该自动化系统将有助于使用电生理或不对称脂质体分析对膜蛋白活性和功能进行高通量研究。
Artificial cell membranes such as liposomes and lipid vesicles have been developed using microfluidic technologies. These technologies have advantages for generating cell-sized liposomes, including a monodispersed and asymmetric lipid composition. Cell-sized asymmetric liposomes with various combinations of asymmetric lipid leaflets are sequentially produced using an improved pulsed-jetting device with the six wells arranged on a circular rotation table by manual manipulation. In this study, we reveal optimal parameters of rotation speed and jetting timing for the cell-sized liposome formation using an automatic sequential liposome generation system driven by a stepping motor. First, we optimize the rotation speed of the stepping motor for forming the planar lipid bilayer under control of a microcomputer. Next, we generate cell-sized liposomes using this automated system at the optimized rotation speed. This automatic system will contribute to high-throughput investigations of membrane protein activities and functions using the electrophysiological or the asymmetric liposome analyses.