Genome characterization and taxonomy of Plantago asiatica mosaic potexvirus.
Genome characterization and taxonomy of Plantago asiatica mosaic potexvirus.
复制标题
车前草花叶病毒的基因组特征和分类。
DOI：
10.1099/0022-1317-75-2-259
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
S. Morozov
中科院分区：
文献类型：
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作者：
Andrey G. Solovyev;V. Novikov;Andres Merits;E. Savenkov;Zelenina Da;L. G. Tyulkina;S. Morozov
测定了车前花叶病毒（Plantagoasiaticamosaicvirus，P1AMV）基因组RNA的全序列。该基因全长6128个核苷酸，含有5个开放阅读框（ORF），分别编码M（r）156 K（ORF 1）、25 K（ORF 2）、12 K（ORF 3）、13 K（ORF 4）和22 K（ORF 5）。这些P1AMV蛋白质的序列表现出很强的同源性，其他马铃薯X病毒的蛋白质。通过对P1AMV ORF1蛋白和衣壳蛋白中3个保守结构域的多重序列比对，构建了P1AMV与番木瓜花叶病毒和三叶草黄花叶病毒的系统进化树。用北方印迹杂交检测到P1AMV基因组RNA和一个0.9kb的主要亚基因组RNA（sgRNA）。后者sgRNA是病毒衣壳蛋白的信使，其5'末端位于外壳蛋白基因的起始密码子上游23个核苷酸处。P1AMV病毒粒子RNA和类似于0.9 kb sgRNA的RNA转录物已在体外翻译，分别产生单个主要170 K产物和主要22 K产物。
The complete nucleotide sequence of Plantago asiatica mosaic virus (P1AMV) genomic RNA has been determined. The 6128 nucleotide sequence contains five open reading frames (ORFs) coding for proteins of M(r) 156K (ORF1), 25K (ORF2), 12K (ORF3), 13K (ORF4) and 22K (ORF5). The sequences of these P1AMV proteins exhibit strong homology to the proteins of the other potexviruses. Phylogenetic trees based on the multiple sequence alignments of three conserved domains in ORF1 product and capsid protein reveal a close relationship of P1AMV to papaya mosaic virus and clover yellow mosaic virus. The P1AMV genomic RNA and a major subgenomic RNA (sgRNA) of 0.9 kb have been detected in infected leaves by Northern blot hybridization. The latter sgRNA is the messenger for virus capsid protein and its 5' terminus has been located 23 nucleotides upstream of the initiator codon of the coat protein gene. The P1AMV virion RNA and RNA transcript resembling the 0.9 kb sgRNA have been translated in vitro giving rise to a single major 170K product and a major 22K product, respectively.