Helicobacter suis KB1 derived from pig gastric lymphoid follicles induces the formation of gastric lymphoid follicles in mice through the activation of B cells and CD4 positive cells
Helicobacter suis KB1 derived from pig gastric lymphoid follicles induces the formation of gastric lymphoid follicles in mice through the activation of B cells and CD4 positive cells
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DOI：
10.1016/j.micinf.2011.02.007
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发表时间：
2011-07-01
影响因子：
5.8
通讯作者：
Azuma, Takeshi
中科院分区：
文献类型：
--
作者：
Yamamoto, Koji;Tanaka, Hiroshi;Azuma, Takeshi
“Helicobacter heilmannii”（“H. heilmannii”）属于幽门螺杆菌属，是一组表现出长螺旋形形态的细菌物种。最近的研究表明，“heilmannii”I型实际上是猪嗜血杆菌，主要寄生在各种动物和人类的胃里。然而，猪嗜血杆菌对胃疾病的影响尚不完全清楚。在本报告中，我们揭示了猪胃中天然猪嗜血杆菌感染与滤泡性胃炎之间的关系。通过16S rRNA、脲酶A和脲酶B基因序列分析，证实猪嗜血杆菌存在于猪胃淋巴滤泡中，并将其命名为猪嗜血杆菌KB1。此外，将猪嗜血杆菌KB1接种于C57BL/6J小鼠，建立猪嗜血杆菌感染致滤泡性胃炎的小鼠模型：猪嗜血杆菌KB1在小鼠胃内定植，诱导淋巴滤泡发育，获得以B细胞和CD4阳性细胞活化为特征的免疫应答。这些结果可能有助于更好地理解猪嗜血杆菌与胃疾病，特别是滤泡性胃炎的关系；此外，我们的研究结果强调了猪嗜血杆菌动物-人感染的人畜共患方面。(C) 2011年巴斯德研究所。Elsevier Masson SAS出版。版权所有。
"Helicobacter heilmannii" ("H. heilmannii"), which belongs to the genus Helicobacter, is a group of bacterial species that display a long spiral-shaped morphology. Recent studies have demonstrated that "H. heilmannii" type I is actually H. suis, which mainly colonizes the stomachs of various animals and humans. However, the influence of H. suis on gastric diseases remains to be fully elucidated. In this report, we revealed the relationship between natural H. suis infection and follicular gastritis in the pig stomachs. From sequence analysis of the 16S rRNA, urease A, and urease B genes, the presence of H. suis was confirmed in pig gastric lymphoid follicles, and this bacterium was named H. suis KB1. In addition, H. suis KB1 was inoculated into C57BL/6J mice, and the following mouse model of the pathogenesis of follicular gastritis by H. suis infection was established: H. suis KB1 colonizes the mouse stomach, and moreover, induces the development of lymphoid follicles and acquired immune responses characterized by the activation of B cells and CD4 positive cells. These results may lead to better understanding of the relationship between H. suis and gastric diseases, especially follicular gastritis; and furthermore, our findings emphasize the zoonotic aspects of animal-human infection by H. suis. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.