THE MIDLINE OF THE DROSOPHILA CENTRAL-NERVOUS-SYSTEM - A MODEL FOR THE GENETIC-ANALYSIS OF CELL FATE, CELL-MIGRATION, AND GROWTH CONE GUIDANCE
THE MIDLINE OF THE DROSOPHILA CENTRAL-NERVOUS-SYSTEM - A MODEL FOR THE GENETIC-ANALYSIS OF CELL FATE, CELL-MIGRATION, AND GROWTH CONE GUIDANCE
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DOI：
10.1016/0092-8674(91)90509-w
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发表时间：
1991-02-22
期刊：
影响因子：
64.5
通讯作者：
GOODMAN, CS
中科院分区：
文献类型：
--
作者：
KLAMBT, C;JACOBS, JR;GOODMAN, CS
一排中胚层细胞将果蝇胚胎的两个侧面神经发生区分开，并产生一组离散的胶质细胞和神经元。大多数中枢神经系统生长锥体最初直接朝向中线，这表明这些中线细胞在轴突连合的形成中起着关键作用。我们使用了第一轴突染色的抗体，选择性染色不同中线细胞的β-半乳糖苷酶增强子陷阱线，以及电子显微镜研究来阐明细胞和相互作用，这些细胞和相互作用介导了每个节段两个主要连合的正常形成。这一分析导致了一个模型，该模型提出了一系列控制轴突连合发展的顺序细胞相互作用。这一模型的基因测试利用了许多突变，这些突变通过消除或改变各种中线细胞的分化，以一种可预测的方式扰乱轴突连合的发育。
A row of mesectodermal cells separates the two lateral neurogenic regions in the Drosophila embryo and generates a discrete set of glia and neurons. Most CNS growth cones initially head straight toward the midline, suggesting that these midline cells play a key role in the formation of the axon commissures. We have used antibodies that stain the first axons, beta-galactosidase enhancer trap lines that selectively stain the different midline cells, and electron microscopic studies to elucidate the cells and interactions that mediate the normal formation of the two major commissures in each segment. This analysis has led to a model that proposes a series of sequential cell interactions controlling the development of the axon commissures. A genetic test of this model has utilized a number of mutations that, by either eliminating or altering the differentiation of various midline cells, perturb the development of the axon commissures in a predictable fashion.