Possibilities and limitations of label-free detection of DNA hybridization with field-effect-based devices
Possibilities and limitations of label-free detection of DNA hybridization with field-effect-based devices
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DOI：
10.1016/j.snb.2005.03.083
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发表时间：
2005-11-11
影响因子：
8.4
通讯作者：
Schöning, MJ
中科院分区：
文献类型：
--
作者：
Poghossian, A;Cherstvy, A;Schöning, MJ
一个关键的评价的可能性和限制的无标记检测脱氧核糖核酸（DNA）杂交的装置场效应为基础的设备进行了讨论。介绍了一种新的DNA检测方法，它利用离子敏感场效应器件作为传感器。提出了DNA杂交诱导的分子间空间内离子浓度的重新分布和/或装置的离子灵敏度的改变作为检测机制。理论计算预测杂交后诱导的分子间空间内的平均离子浓度的实质性变化足以获得可检测的传感器信号。(c)2005 Elsevier B. V.保留所有权利。
A critical evaluation of the possibilities and limitations of the label-free detection of deoxyribonucleic acid (DNA) hybridization by means of field-effect-based devices is discussed. A new DNA-detection method is introduced, which utilizes an ion-sensitive field-effect device as transducer. The upon the DNA hybridization induced redistribution of the ion concentration within the intermolecular spaces and/or the alteration of the ion sensitivity of the device is proposed as detection mechanism. The theoretical calculations predict a substantial change in the average ion concentration within the intermolecular spaces induced upon hybridization that are enough to obtain a detectable sensor signal. (c) 2005 Elsevier B.V. All rights reserved.